Inquiry into mechanism of FDP accumulating using model of metabolic pathway flux

In this paper the model of metabolic pathway flux of FDP accumulation was establish and the metabolic flux distributions were obtained at different times of FDP accumulation process. The experiments show that FDP accumulation in the medium produced by the present method is due to the redirected flux distributions in the metabolic pathway. The yield of FDP to glucose was only 16.1%. Meanwhile, the results also showed that such metabolic flux alterations at key branch points are often directly opposed by mechanisms for controlling system of the enzyme activity, such as HK, PFK, PK, et al, which have evolved to maintain flux distributions for an optimal growth. So, in order to overproduce FDP with yeasts, enzyme amplification or other modifications of the production pathway must be done to alter the metabolic flux distributions at the key branch points.