Abstract
Rapid and reliable detection of pathogenic bacteria is crucial for food safety control. Here, we present a novel luciferase-zinc finger system for the detection of pathogens that offers rapid and specific profiling. The system, which uses a zinc-finger protein domain to probe zinc finger recognition sites, was designed to bind the amplified conserved regions of 16S rDNA, and the obtained products were detected using a modified luciferase. The luciferase-zinc finger system not only maintained luciferase activity but also allowed the specific detection of different bacterial species, with a sensitivity as low as 10 copies and a linear range from 10 to 104 copies per microliter of the specific PCR product. Moreover, the system is robust and rapid, enabling the simultaneous detection of 6 species of bacteria in artificially contaminated samples with excellent accuracy. Thus, we envision that our luciferase-zinc finger system will have far-reaching applications.
Original language | English |
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Pages (from-to) | 6674-6681 |
Number of pages | 8 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 65 |
Issue number | 31 |
DOIs | |
State | Published - 9 Aug 2017 |
Keywords
- detection
- luciferase
- pathogenic bacteria
- zinc-finger protein