Mesoporous material SBA-15 modified by amino acid ionic liquid to immobilize lipase via ionic bonding and cross-linking method

Novel supported task-specific amino acid ionic liquids have been developed for the first time via the ionic-pair coupling of imidazolium cation of the modified mesoporous material SBA-15 with l-lysine anion. The material functionalized by amino acid ionic liquid (AA-SBA) as a novel carrier was used to immobilize porcine pancreas lipase (PPL) through ionic bonding interaction and cross-linking method (PPL-AA-SBA). To investigate the stability changes of the lipase immobilized onto novel carrier AA-SBA via special methods, the enzymatic properties of PPL-AA-SBA were studied in the triacetin hydrolysis reaction. The results indicated that the immobilization efficiency of PPL was up to 98% and the stability of lipase immobilized onto AA-SBA was dramatically improved. Especially the leaching investigation in consecutive use showed that special immobilization method inhibited the enzyme leaching obviously, and immobilization efficiency was above 96% after five recycles.