TY - JOUR
T1 - Multi- and transcriptomic changes of chronic exposure to bisphenol A reveals reproductive toxicity in male zebrafish
AU - Luo, Xu
AU - Du, Zhanxiang
AU - Hu, Jinyuan
AU - Retyunskiy, Vladimir
AU - Ma, Bo
AU - Liu, Shan
AU - Gao, Xing
AU - Zhao, Ye
AU - Zhang, Qi
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2023/8
Y1 - 2023/8
N2 - Bisphenol A (BPA) is considered to be a threat to marine organisms owning to its widespread usage and potential aquatic toxicity. However, the reproductive toxicity of BPA to transgenerational inheritance in aquatic organisms is still unclarified. In this study, the morphological, histological, and transgenerational changes by BPA in zebrafish testis were investigated. Results showed that BPA caused abnormities in sperm number, activity, and fertility rate. Testicular transcriptional alterations detected by RNA-seq identified 1940 differentially expressed genes (DEGs) after BPA exposure, of which 392 were upregulated and 1548 were downregulated. Gene Ontology analysis showed that acrosin binding, binding of sperm to zona pellucida, and positive regulation of acrosome reaction were significantly enriched in BPA-induced DEGs. Pathway analysis indicated that cell adhesion molecules, steroid hormone biosynthesis and fatty acid biosynthesis, elongation, and metabolism were remarkably changed after BPA treatment. Thus, we deduce here that multi- and transcriptomic changes of chronic exposure to BPA reveals reproductive toxicity in male zebrafish.
AB - Bisphenol A (BPA) is considered to be a threat to marine organisms owning to its widespread usage and potential aquatic toxicity. However, the reproductive toxicity of BPA to transgenerational inheritance in aquatic organisms is still unclarified. In this study, the morphological, histological, and transgenerational changes by BPA in zebrafish testis were investigated. Results showed that BPA caused abnormities in sperm number, activity, and fertility rate. Testicular transcriptional alterations detected by RNA-seq identified 1940 differentially expressed genes (DEGs) after BPA exposure, of which 392 were upregulated and 1548 were downregulated. Gene Ontology analysis showed that acrosin binding, binding of sperm to zona pellucida, and positive regulation of acrosome reaction were significantly enriched in BPA-induced DEGs. Pathway analysis indicated that cell adhesion molecules, steroid hormone biosynthesis and fatty acid biosynthesis, elongation, and metabolism were remarkably changed after BPA treatment. Thus, we deduce here that multi- and transcriptomic changes of chronic exposure to BPA reveals reproductive toxicity in male zebrafish.
KW - Bisphenol A
KW - RNA-Seq
KW - Sperm
KW - Testis
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85164572094&partnerID=8YFLogxK
U2 - 10.1007/s10695-023-01214-4
DO - 10.1007/s10695-023-01214-4
M3 - 文章
C2 - 37436566
AN - SCOPUS:85164572094
SN - 0920-1742
VL - 49
SP - 671
EP - 685
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
IS - 4
ER -
