Optimisation of the ethanol-based heat reflux extraction of isoflavones from Pueraria lobata (Willd.) Ohwi root

Shu Ya Wang, Jun Gao, Yi Lu Chen, Jun Zhou, Xiao Yu Yong, Xiao Ning Liu, Ya Bing Zhang, Xiao Feng Liu, Yong Ming Sun, Tao Zheng

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations

Abstract

Ethanol-based heat reflux extraction has been successfully applied in extracting isoflavone compounds from the dried root of Pueraria lobata (Willd.) Ohwi. The different extraction factors including ethanol concentration, extraction temperature, extraction time, ratio of solid/liquid and number of extraction cycles has been studied on the influence of the extraction yield of isoflavone compounds. And the orthogonal experiment has been used to optimize the extracting conditions. A variety of indicators were employed to evaluate the extraction efficiency, including the yield of puerarin, daidzein and total isoflavone. The optimal extraction technology was as follows: extraction temperature 90°C, ratio of solid/liquid 1:6, ethanol concentration 90% and extraction 2 h. Under the optimal conditions extraction rates of puerarin, daidzein and total isoflavone were 37.51±0.64 mg/g, 2.63±0.03 mg/g and 117.4±0.89 mg/g. The study of this article can provide theoretical basis for large-scale production of puerarin, daidzein and total isoflavone.

Original languageEnglish
Title of host publicationAdvances in Environmental Technologies III
PublisherTrans Tech Publications Ltd
Pages180-186
Number of pages7
ISBN (Print)9783038351351
DOIs
StatePublished - 2014
Event3rd International Conference on Energy and Environmental Protection, ICEEP 2014 - Xi'an, China
Duration: 26 Apr 201428 Apr 2014

Publication series

NameAdvanced Materials Research
Volume955-959
ISSN (Print)1022-6680
ISSN (Electronic)1662-8985

Conference

Conference3rd International Conference on Energy and Environmental Protection, ICEEP 2014
Country/TerritoryChina
CityXi'an
Period26/04/1428/04/14

Keywords

  • Daidzein
  • HPLC
  • Heat reflux extraction
  • Puerarin
  • Ttotal isoflavone

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