TY - JOUR
T1 - Optimization of culture conditions for enhanced lysine production using engineered Escherichia coli
AU - Ying, Hanxiao
AU - He, Xun
AU - Li, Yan
AU - Chen, Kequan
AU - Ouyang, Pingkai
PY - 2014/4
Y1 - 2014/4
N2 - In this study, culture conditions, including dissolved oxygen (DO) content, presence of osmoprotectants, residual glucose concentration, and ammonium sulfate-feeding strategies, were investigated for decreasing the inhibition effects of acetic acid, ammonium, and osmotic stress on L-lysine fermentation by Escherichia coli. The results revealed that higher DO content and lower residual glucose concentration could decrease acetic acid accumulation, betaine supplementation could enhance osmotic stress tolerance, and variable speed ammonium sulfate-feeding strategy could decrease ammonium inhibition. Thus, with 25 % DO content, 0-5.0 g/L of residual glucose concentration, and 1.5 g/L of betaine supplementation, 134.9 g/L of L-lysine was obtained after 72 h of culture, with L-lysine yield and productivity of 45.4 % and 1.9 g/(L·h), respectively.
AB - In this study, culture conditions, including dissolved oxygen (DO) content, presence of osmoprotectants, residual glucose concentration, and ammonium sulfate-feeding strategies, were investigated for decreasing the inhibition effects of acetic acid, ammonium, and osmotic stress on L-lysine fermentation by Escherichia coli. The results revealed that higher DO content and lower residual glucose concentration could decrease acetic acid accumulation, betaine supplementation could enhance osmotic stress tolerance, and variable speed ammonium sulfate-feeding strategy could decrease ammonium inhibition. Thus, with 25 % DO content, 0-5.0 g/L of residual glucose concentration, and 1.5 g/L of betaine supplementation, 134.9 g/L of L-lysine was obtained after 72 h of culture, with L-lysine yield and productivity of 45.4 % and 1.9 g/(L·h), respectively.
KW - Engineered E. coli
KW - Fermentation
KW - Inhibition
KW - L-lysine
KW - Optimization
UR - http://www.scopus.com/inward/record.url?scp=84899463616&partnerID=8YFLogxK
U2 - 10.1007/s12010-014-0820-7
DO - 10.1007/s12010-014-0820-7
M3 - 文章
C2 - 24682878
AN - SCOPUS:84899463616
SN - 0273-2289
VL - 172
SP - 3835
EP - 3843
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 8
ER -