TY - JOUR
T1 - Photo-crosslinkable hyaluronic acid microgels with reactive oxygen species scavenging capacity for mesenchymal stem cell encapsulation
AU - Hao, Shiqi
AU - Tian, Chen
AU - Bai, Yimeng
AU - Wu, Lihuang
AU - Hao, Lili
AU - Kuang, Yi
AU - Yang, Shengxiang
AU - Mao, Hongli
AU - Gu, Zhongwei
N1 - Publisher Copyright:
© 2023
PY - 2023/7/15
Y1 - 2023/7/15
N2 - Mesenchymal stem cells (MSCs) have gained increasing attention in various biomedical applications. However, conventional therapeutic approaches, such as direct intravenous injection, are associated with low cell survival due to the shear force during injection and the oxidative stress microenvironments in the lesion area. Herein, a photo-crosslinkable antioxidant hydrogel based on tyramine- and dopamine-modified hyaluronic acid (HA-Tyr/HA-DA) was developed. Meanwhile, human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were encapsulated in HA-Tyr/HA-DA hydrogel using a microfluidic system to create size-controllable microgels (hUC-MSCs@microgels). The HA-Tyr/HA-DA hydrogel was demonstrated to have good rheology, biocompatibility, and antioxidant properties for cell microencapsulation. The hUC-MSCs encapsulated in microgels showed a high viability and a significantly improved the survival rate under oxidative stress conditions. Therefore, the presented work provides a promising platform for MSCs microencapsulation, which may further improve the stem cell-based biomedical applications.
AB - Mesenchymal stem cells (MSCs) have gained increasing attention in various biomedical applications. However, conventional therapeutic approaches, such as direct intravenous injection, are associated with low cell survival due to the shear force during injection and the oxidative stress microenvironments in the lesion area. Herein, a photo-crosslinkable antioxidant hydrogel based on tyramine- and dopamine-modified hyaluronic acid (HA-Tyr/HA-DA) was developed. Meanwhile, human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were encapsulated in HA-Tyr/HA-DA hydrogel using a microfluidic system to create size-controllable microgels (hUC-MSCs@microgels). The HA-Tyr/HA-DA hydrogel was demonstrated to have good rheology, biocompatibility, and antioxidant properties for cell microencapsulation. The hUC-MSCs encapsulated in microgels showed a high viability and a significantly improved the survival rate under oxidative stress conditions. Therefore, the presented work provides a promising platform for MSCs microencapsulation, which may further improve the stem cell-based biomedical applications.
KW - Photo-crosslinkable microgel
KW - ROS scavenging
KW - Stem cell encapsulation
UR - http://www.scopus.com/inward/record.url?scp=85161651693&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.124971
DO - 10.1016/j.ijbiomac.2023.124971
M3 - 文章
C2 - 37236562
AN - SCOPUS:85161651693
SN - 0141-8130
VL - 243
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 124971
ER -