Plasma treatment of multi-walled carbon nanotubes for lipase immobilization

Xun Cao; Rui Zhang; Wei min Tan; Ce Wei; Jing Wang; Ze meng Liu; Ke quan Chen; Ping kai Ouyang

doi:10.1007/s11814-016-0002-0

Plasma treatment of multi-walled carbon nanotubes for lipase immobilization

Xun Cao, Rui Zhang, Wei min Tan, Ce Wei, Jing Wang, Ze meng Liu, Ke quan Chen, Ping kai Ouyang

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Plasma-modified multiwalled carbon nanotubes (MWNTs) were used as a support to immobilize lipase. The effects of vacuum plasma treatment power, vacuum plasma treatment time, immobilization temperature, immobilization time, and initial protein concentration of the lipase on the amount of lipase immobilized and on the subsequent activity of the immobilized lipase were investigated. The results showed that the adsorption capacity of the plasma-modified MWNTs could reach 0.15 g/g and that the maximal enzyme activity of the immobilized lipase was 520U/g under optimized conditions. Fourier transform infrared (FTIR) analysis and transmission electron microscopy (TEM) were used to characterize the properties of the plasma-modified MWNTs and plasma-modified MWNTs-lipase, and the results showed that the lipase was successfully immobilized on the plasma-modified MWNTs. Also, the MWNTs-lipase produced an esterification rate of approximately 47% in the synthesis of polyethylene glycol (PEG)-aliphatic esters.

Original language	English
Pages (from-to)	1653-1658
Number of pages	6
Journal	Korean Journal of Chemical Engineering
Volume	33
Issue number	5
DOIs	https://doi.org/10.1007/s11814-016-0002-0
State	Published - 1 May 2016

Keywords

Esterification
Lipase
Multiwalled Carbon Nanotubes
Plasma-modified

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10.1007/s11814-016-0002-0

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title = "Plasma treatment of multi-walled carbon nanotubes for lipase immobilization",

abstract = "Plasma-modified multiwalled carbon nanotubes (MWNTs) were used as a support to immobilize lipase. The effects of vacuum plasma treatment power, vacuum plasma treatment time, immobilization temperature, immobilization time, and initial protein concentration of the lipase on the amount of lipase immobilized and on the subsequent activity of the immobilized lipase were investigated. The results showed that the adsorption capacity of the plasma-modified MWNTs could reach 0.15 g/g and that the maximal enzyme activity of the immobilized lipase was 520U/g under optimized conditions. Fourier transform infrared (FTIR) analysis and transmission electron microscopy (TEM) were used to characterize the properties of the plasma-modified MWNTs and plasma-modified MWNTs-lipase, and the results showed that the lipase was successfully immobilized on the plasma-modified MWNTs. Also, the MWNTs-lipase produced an esterification rate of approximately 47% in the synthesis of polyethylene glycol (PEG)-aliphatic esters.",

keywords = "Esterification, Lipase, Multiwalled Carbon Nanotubes, Plasma-modified",

author = "Xun Cao and Rui Zhang and Tan, {Wei min} and Ce Wei and Jing Wang and Liu, {Ze meng} and Chen, {Ke quan} and Ouyang, {Ping kai}",

note = "Publisher Copyright: {\textcopyright} 2016, Korean Institute of Chemical Engineers, Seoul, Korea.",

year = "2016",

month = may,

day = "1",

doi = "10.1007/s11814-016-0002-0",

language = "英语",

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TY - JOUR

T1 - Plasma treatment of multi-walled carbon nanotubes for lipase immobilization

AU - Cao, Xun

AU - Zhang, Rui

AU - Tan, Wei min

AU - Wei, Ce

AU - Wang, Jing

AU - Liu, Ze meng

AU - Chen, Ke quan

AU - Ouyang, Ping kai

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Plasma-modified multiwalled carbon nanotubes (MWNTs) were used as a support to immobilize lipase. The effects of vacuum plasma treatment power, vacuum plasma treatment time, immobilization temperature, immobilization time, and initial protein concentration of the lipase on the amount of lipase immobilized and on the subsequent activity of the immobilized lipase were investigated. The results showed that the adsorption capacity of the plasma-modified MWNTs could reach 0.15 g/g and that the maximal enzyme activity of the immobilized lipase was 520U/g under optimized conditions. Fourier transform infrared (FTIR) analysis and transmission electron microscopy (TEM) were used to characterize the properties of the plasma-modified MWNTs and plasma-modified MWNTs-lipase, and the results showed that the lipase was successfully immobilized on the plasma-modified MWNTs. Also, the MWNTs-lipase produced an esterification rate of approximately 47% in the synthesis of polyethylene glycol (PEG)-aliphatic esters.

AB - Plasma-modified multiwalled carbon nanotubes (MWNTs) were used as a support to immobilize lipase. The effects of vacuum plasma treatment power, vacuum plasma treatment time, immobilization temperature, immobilization time, and initial protein concentration of the lipase on the amount of lipase immobilized and on the subsequent activity of the immobilized lipase were investigated. The results showed that the adsorption capacity of the plasma-modified MWNTs could reach 0.15 g/g and that the maximal enzyme activity of the immobilized lipase was 520U/g under optimized conditions. Fourier transform infrared (FTIR) analysis and transmission electron microscopy (TEM) were used to characterize the properties of the plasma-modified MWNTs and plasma-modified MWNTs-lipase, and the results showed that the lipase was successfully immobilized on the plasma-modified MWNTs. Also, the MWNTs-lipase produced an esterification rate of approximately 47% in the synthesis of polyethylene glycol (PEG)-aliphatic esters.

KW - Esterification

KW - Lipase

KW - Multiwalled Carbon Nanotubes

KW - Plasma-modified

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U2 - 10.1007/s11814-016-0002-0

DO - 10.1007/s11814-016-0002-0

M3 - 文章

AN - SCOPUS:84961150233

SN - 0256-1115

VL - 33

SP - 1653

EP - 1658

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

IS - 5

ER -

Plasma treatment of multi-walled carbon nanotubes for lipase immobilization

Abstract

Keywords

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