TY - JOUR
T1 - Preparative isolation and purification of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. by high-speed counter-current chromatography
AU - Yan, Guilong
AU - Ji, Lilian
AU - Luo, Yuming
AU - Hu, Yonghong
PY - 2012/8
Y1 - 2012/8
N2 - A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxytigloyloxy]- costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H-NMR.
AB - A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxytigloyloxy]- costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H-NMR.
KW - Eupatorium lindleyanum DC
KW - High-speed counter-current chromatography
KW - Separation
KW - Sesquiterpenoid lactone
UR - http://www.scopus.com/inward/record.url?scp=84865483782&partnerID=8YFLogxK
U2 - 10.3390/molecules17089002
DO - 10.3390/molecules17089002
M3 - 文章
C2 - 22842644
AN - SCOPUS:84865483782
SN - 1420-3049
VL - 17
SP - 9002
EP - 9009
JO - Molecules
JF - Molecules
IS - 8
ER -