Pretreatment of Luzhou Distiller’s Grains with Crude Enzyme from Trichoderma harzianum for Feed Protein Production

This study developed a solid-state fermentation system based on Trichoderma harzianum, which significantly enhanced the nutritional value of distiller’s grain (DG) feed through a multi-stage synergistic treatment process. During the cellulase production phase, rice husk was used as an auxiliary material, and specific degradation of DGs was effectively enhanced. Through optimization using response surface methodology, the optimal enzyme production conditions were determined. The filter paper enzyme activity reached a peak of 1.45 U/gds (enzyme activity per gram of dry substrate) when the moisture content was 53%, the fermentation time was 3 days, and the Tween-80 dosage was 0.015 mL/g (dry weight basis). Under these conditions, the crude enzyme solution was used to hydrolyze DGs. Compared to original DGs, the content of reducing sugars increased by 10.75%. In the stage of protein production, segmented hydrolysis fermentation (SHF) and simultaneous saccharification fermentation (SSF) processes were employed using yeast. The results showed that SSF pathway showed better performance, and the true protein content reached 15.16% after 11 days, an increase of 41.5% compared to the control. Finally, through secondary fermentation regulated by Lactobacillus fermentum, the flavor of the feed was significantly improved. This study innovatively integrated bio-enzymatic hydrolysis and multi-strain synergistic fermentation technologies, providing a novel strategy for the efficient and sustainable production of protein feed based on DGs.