Production of rHuEPO via culturing CHO cells on high efficient and cheap culture carrier

The processes of culturing CHO(Chinese Hamster Ovary)cells to produce rHuEPO(recombinant human Erythropoietin)were investigated by using self-prepared collagen carrier, self-prepared C-DISK carrier and imported A-DISK carrier, respectively. The analysis and comparison of the anchorage of CHO cells, metabolic rates of glucose, expression concentration of rHuEPO and the relation between metabolic rate of glucose and expression concentration of rHuEPO for culturing CHO cells on three different carriers were experimentally carried out. The results show that self-prepared collagen carrier has the best bio-activity and highest protein expression in CHO cell culture. The maximum glucose metabolic rate of CHO cells cultured on self-prepared collagen carrier is as high as 14.2 mmol·mL^-1·h^-1, and which is 16.4% higher than that on C-DISK carrier, 12.8% higher than that on A-DISK carrier, the final rHuEPO expression concentration in CHO cell culture cultured on self-prepared collagen carriers reaches 282 U·mL^-1 after culturing ten days, and it is 30% higher than that on C-DISK carrier, 18% higher than that on A-DISK carrier. The self-prepared collagen carrier is a kind of high efficient and cheaper carrier which can be used for large-scale animal cell culture. The experimental results also show that the metabolic rate of glucose and expression concentration of rHuEPO in CHO cell culture cultured on different carriers are strongly relevant, but they are not in synchronization. This means that CHO cell culture stage can be detected and regulated by measuring the change of glucose metabolic rate.