Purification and structural characterization of ε-PL produced by Kitasatospora sp. PL6-3

Jun Zhou; Hong Xu; Jun Wang; Zhong Yao; Hui Wang; Pingkai Ouyang

Purification and structural characterization of ε-PL produced by Kitasatospora sp. PL6-3

Jun Zhou, Hong Xu, Jun Wang, Zhong Yao, Hui Wang, Pingkai Ouyang

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

ε-Poly-L-lysine (ε-PL) was isolated and purified from the culture broth of Kitasatospora sp. PL6-3. Through the ion exchange process and Sephadex G-25 gel filtration chromatography, a high purity product was obtained with overall yield of 81.2%. The purified ε-PL was characterized with UV, IR and HR/MAS NMR. Its relative molecular weights as determined by SDS-PAGE and gel permeation chromatography (GPC) were about 5.01 × 10³ and 5.05 × 10³ respectively, which was higher than the other ε-PL reported before.

Original language	English
Pages (from-to)	1957-1961
Number of pages	5
Journal	Huagong Xuebao/CIESC Journal
Volume	57
Issue number	8
State	Published - Aug 2006

Keywords

Purification
Structural characterization
ε-poly-L-lysine

Cite this

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title = "Purification and structural characterization of ε-PL produced by Kitasatospora sp. PL6-3",

abstract = "ε-Poly-L-lysine (ε-PL) was isolated and purified from the culture broth of Kitasatospora sp. PL6-3. Through the ion exchange process and Sephadex G-25 gel filtration chromatography, a high purity product was obtained with overall yield of 81.2%. The purified ε-PL was characterized with UV, IR and HR/MAS NMR. Its relative molecular weights as determined by SDS-PAGE and gel permeation chromatography (GPC) were about 5.01 × 103 and 5.05 × 103 respectively, which was higher than the other ε-PL reported before.",

keywords = "Purification, Structural characterization, ε-poly-L-lysine",

author = "Jun Zhou and Hong Xu and Jun Wang and Zhong Yao and Hui Wang and Pingkai Ouyang",

year = "2006",

month = aug,

language = "英语",

volume = "57",

pages = "1957--1961",

journal = "Huagong Xuebao/CIESC Journal",

issn = "0438-1157",

publisher = "Materials China",

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TY - JOUR

T1 - Purification and structural characterization of ε-PL produced by Kitasatospora sp. PL6-3

AU - Zhou, Jun

AU - Xu, Hong

AU - Wang, Jun

AU - Yao, Zhong

AU - Wang, Hui

AU - Ouyang, Pingkai

PY - 2006/8

Y1 - 2006/8

N2 - ε-Poly-L-lysine (ε-PL) was isolated and purified from the culture broth of Kitasatospora sp. PL6-3. Through the ion exchange process and Sephadex G-25 gel filtration chromatography, a high purity product was obtained with overall yield of 81.2%. The purified ε-PL was characterized with UV, IR and HR/MAS NMR. Its relative molecular weights as determined by SDS-PAGE and gel permeation chromatography (GPC) were about 5.01 × 103 and 5.05 × 103 respectively, which was higher than the other ε-PL reported before.

AB - ε-Poly-L-lysine (ε-PL) was isolated and purified from the culture broth of Kitasatospora sp. PL6-3. Through the ion exchange process and Sephadex G-25 gel filtration chromatography, a high purity product was obtained with overall yield of 81.2%. The purified ε-PL was characterized with UV, IR and HR/MAS NMR. Its relative molecular weights as determined by SDS-PAGE and gel permeation chromatography (GPC) were about 5.01 × 103 and 5.05 × 103 respectively, which was higher than the other ε-PL reported before.

KW - Purification

KW - Structural characterization

KW - ε-poly-L-lysine

UR - http://www.scopus.com/inward/record.url?scp=33749832974&partnerID=8YFLogxK

M3 - 文章

AN - SCOPUS:33749832974

SN - 0438-1157

VL - 57

SP - 1957

EP - 1961

JO - Huagong Xuebao/CIESC Journal

JF - Huagong Xuebao/CIESC Journal

IS - 8

ER -

Purification and structural characterization of ε-PL produced by Kitasatospora sp. PL6-3

Abstract

Keywords

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