Regulate properties of glucose oxidase biosensors through pore sizes of TiO2 nanotube arrays

Chao Rao; Yihui Dong; Wei Zhuang; Xinbing Wu; Qiliang Hong; Chang Liu; Xiaohua Lu

doi:10.11949/j.issn.0438-1157.20160181

Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays

Chao Rao, Yihui Dong, Wei Zhuang, Xinbing Wu, Qiliang Hong, Chang Liu, Xiaohua Lu

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Highly ordered TiO₂ nanotube arrays (TNA) with controlled pore sizes in a series of 21, 62, 83 and 102 nm were synthesized using constant current oxidization method. Glucose oxidases (GOx) were immobilized on TNA by physical adsorption, and the GOx activities on TNA were investigated by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy. All immobilized GOx had good oxidizing activities in glucose solution and GOx on 83 nm-pore-sized TNA showed the best sensibility of 27.2 μA·(mmol·L^-1)^-1·cm^-2, which was probably due to a combination effect of reduced diffusion resistance and small Michaelis constant of the immobilized GOx. The experimental results had demonstrated that controlling pore sizes of TNA could effectively tune sensitivity of glucose biosensors.

Original language	English
Pages (from-to)	4324-4333
Number of pages	10
Journal	Huagong Xuebao/CIESC Journal
Volume	67
Issue number	10
DOIs	https://doi.org/10.11949/j.issn.0438-1157.20160181
State	Published - 1 Oct 2016

Keywords

Bioprocess
Biosensor
Diffusion resistance
Electrochemistry
Glucose oxidase
Pore size
Preparation
TiO nanotube arrays

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10.11949/j.issn.0438-1157.20160181

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title = "Regulate properties of glucose oxidase biosensors through pore sizes of TiO2 nanotube arrays",

abstract = "Highly ordered TiO2 nanotube arrays (TNA) with controlled pore sizes in a series of 21, 62, 83 and 102 nm were synthesized using constant current oxidization method. Glucose oxidases (GOx) were immobilized on TNA by physical adsorption, and the GOx activities on TNA were investigated by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy. All immobilized GOx had good oxidizing activities in glucose solution and GOx on 83 nm-pore-sized TNA showed the best sensibility of 27.2 μA·(mmol·L-1)-1·cm-2, which was probably due to a combination effect of reduced diffusion resistance and small Michaelis constant of the immobilized GOx. The experimental results had demonstrated that controlling pore sizes of TNA could effectively tune sensitivity of glucose biosensors.",

keywords = "Bioprocess, Biosensor, Diffusion resistance, Electrochemistry, Glucose oxidase, Pore size, Preparation, TiO nanotube arrays",

author = "Chao Rao and Yihui Dong and Wei Zhuang and Xinbing Wu and Qiliang Hong and Chang Liu and Xiaohua Lu",

year = "2016",

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doi = "10.11949/j.issn.0438-1157.20160181",

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T1 - Regulate properties of glucose oxidase biosensors through pore sizes of TiO2 nanotube arrays

AU - Rao, Chao

AU - Dong, Yihui

AU - Zhuang, Wei

AU - Wu, Xinbing

AU - Hong, Qiliang

AU - Liu, Chang

AU - Lu, Xiaohua

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Highly ordered TiO2 nanotube arrays (TNA) with controlled pore sizes in a series of 21, 62, 83 and 102 nm were synthesized using constant current oxidization method. Glucose oxidases (GOx) were immobilized on TNA by physical adsorption, and the GOx activities on TNA were investigated by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy. All immobilized GOx had good oxidizing activities in glucose solution and GOx on 83 nm-pore-sized TNA showed the best sensibility of 27.2 μA·(mmol·L-1)-1·cm-2, which was probably due to a combination effect of reduced diffusion resistance and small Michaelis constant of the immobilized GOx. The experimental results had demonstrated that controlling pore sizes of TNA could effectively tune sensitivity of glucose biosensors.

AB - Highly ordered TiO2 nanotube arrays (TNA) with controlled pore sizes in a series of 21, 62, 83 and 102 nm were synthesized using constant current oxidization method. Glucose oxidases (GOx) were immobilized on TNA by physical adsorption, and the GOx activities on TNA were investigated by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy. All immobilized GOx had good oxidizing activities in glucose solution and GOx on 83 nm-pore-sized TNA showed the best sensibility of 27.2 μA·(mmol·L-1)-1·cm-2, which was probably due to a combination effect of reduced diffusion resistance and small Michaelis constant of the immobilized GOx. The experimental results had demonstrated that controlling pore sizes of TNA could effectively tune sensitivity of glucose biosensors.

KW - Bioprocess

KW - Biosensor

KW - Diffusion resistance

KW - Electrochemistry

KW - Glucose oxidase

KW - Pore size

KW - Preparation

KW - TiO nanotube arrays

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M3 - 文章

AN - SCOPUS:85092433060

SN - 0438-1157

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JO - Huagong Xuebao/CIESC Journal

JF - Huagong Xuebao/CIESC Journal

IS - 10

ER -

Regulate properties of glucose oxidase biosensors through pore sizes of TiO₂ nanotube arrays

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Keywords

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