Separation of solid phase synthesis thymosin α1 using ion-exchange method

Hui Liu; Zhong Yao; Chengyong Ding; Yun Wu; Yongsen Chen; Yishen Zhu; Xiaoying Xu

Separation of solid phase synthesis thymosin α1 using ion-exchange method

Hui Liu, Zhong Yao, Chengyong Ding, Yun Wu, Yongsen Chen, Yishen Zhu, Xiaoying Xu

COLLEGE OF FOOD SCIENCE AND LIGHT INDUSTRY

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

Abstract

Thymosin alpha1 (Tα1) is a sort of important immune response modifier and widely used in clinic. In this paper, the SOURCE 15Q and DEAE Sepherose FF resin were selected for purification of solid phase synthesis Tα1 using ion-exchange chromatography and several factors that will influence the process were investigated, such as pH value, sample size, elution protocol and variety of resin. The strategy of purification of Tα1 was established by using SOURCE 15Q as ion-exchanger. The purity of product could reach 95.53% and the yield of purified Tα1 was about 60.4%. Purified peptide was analyzed by ESI-MS and the result showed that the product had the same molecular weight as Tα1. This method can satisfy the requirement of purification of Tα1 in large scale with lower cost.

Original language	English
Pages (from-to)	314-321
Number of pages	8
Journal	Lizi Jiaohuan Yu Xifu/Ion Exchange and Adsorption
Volume	23
Issue number	4
State	Published - Aug 2007

Keywords

Ion-exchange
Purification
SOURCE15Q
Thymosin alpha1

Cite this

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abstract = "Thymosin alpha1 (Tα1) is a sort of important immune response modifier and widely used in clinic. In this paper, the SOURCE 15Q and DEAE Sepherose FF resin were selected for purification of solid phase synthesis Tα1 using ion-exchange chromatography and several factors that will influence the process were investigated, such as pH value, sample size, elution protocol and variety of resin. The strategy of purification of Tα1 was established by using SOURCE 15Q as ion-exchanger. The purity of product could reach 95.53% and the yield of purified Tα1 was about 60.4%. Purified peptide was analyzed by ESI-MS and the result showed that the product had the same molecular weight as Tα1. This method can satisfy the requirement of purification of Tα1 in large scale with lower cost.",

keywords = "Ion-exchange, Purification, SOURCE15Q, Thymosin alpha1",

author = "Hui Liu and Zhong Yao and Chengyong Ding and Yun Wu and Yongsen Chen and Yishen Zhu and Xiaoying Xu",

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TY - JOUR

T1 - Separation of solid phase synthesis thymosin α1 using ion-exchange method

AU - Liu, Hui

AU - Yao, Zhong

AU - Ding, Chengyong

AU - Wu, Yun

AU - Chen, Yongsen

AU - Zhu, Yishen

AU - Xu, Xiaoying

PY - 2007/8

Y1 - 2007/8

N2 - Thymosin alpha1 (Tα1) is a sort of important immune response modifier and widely used in clinic. In this paper, the SOURCE 15Q and DEAE Sepherose FF resin were selected for purification of solid phase synthesis Tα1 using ion-exchange chromatography and several factors that will influence the process were investigated, such as pH value, sample size, elution protocol and variety of resin. The strategy of purification of Tα1 was established by using SOURCE 15Q as ion-exchanger. The purity of product could reach 95.53% and the yield of purified Tα1 was about 60.4%. Purified peptide was analyzed by ESI-MS and the result showed that the product had the same molecular weight as Tα1. This method can satisfy the requirement of purification of Tα1 in large scale with lower cost.

AB - Thymosin alpha1 (Tα1) is a sort of important immune response modifier and widely used in clinic. In this paper, the SOURCE 15Q and DEAE Sepherose FF resin were selected for purification of solid phase synthesis Tα1 using ion-exchange chromatography and several factors that will influence the process were investigated, such as pH value, sample size, elution protocol and variety of resin. The strategy of purification of Tα1 was established by using SOURCE 15Q as ion-exchanger. The purity of product could reach 95.53% and the yield of purified Tα1 was about 60.4%. Purified peptide was analyzed by ESI-MS and the result showed that the product had the same molecular weight as Tα1. This method can satisfy the requirement of purification of Tα1 in large scale with lower cost.

KW - Ion-exchange

KW - Purification

KW - SOURCE15Q

KW - Thymosin alpha1

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M3 - 文章

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SN - 1001-5493

VL - 23

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JO - Lizi Jiaohuan Yu Xifu/Ion Exchange and Adsorption

JF - Lizi Jiaohuan Yu Xifu/Ion Exchange and Adsorption

IS - 4

ER -

Separation of solid phase synthesis thymosin α1 using ion-exchange method

Abstract

Keywords

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