Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography-electrospray mass spectrometry for pharmacokinetic studies

Jianguo Sun; Guangji Wang; Wei Wang; Shuai Zhao; Yi Gu; Jinwen Zhang; Mingwen Huang; Fen Shao; Hao Li; Qi Zhang; Haitang Xie

doi:10.1016/j.jpba.2005.03.041

Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography-electrospray mass spectrometry for pharmacokinetic studies

Jianguo Sun, Guangji Wang, Wei Wang, Shuai Zhao, Yi Gu, Jinwen Zhang, Mingwen Huang, Fen Shao, Hao Li, Qi Zhang, Haitang Xie

China Pharmaceutical University

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

To support the pharmacokinetic and bioavailability study of an extended-release loratadine (LOR)/pseudoephedrine sulfate (PES) tablet, a high performance liquid chromatographic-electrospray ionisation-mass spectrometric method (LC-MS) was developed for the simultaneous determination of LOR and PES in human plasma. Diazepam (DP) and phenylpropanolamine (PPA) were used as internal standards for LOR and PES, respectively. Analytes were extracted from alkalized human plasma by liquid/liquid extraction using ethyl ether. Chromatographic separation was performed on an ODS column at flow rate of 0.2 ml/min. The total chromatographic run time was 10.5 min with the retention time of 7.1 min and 6.2 min for LOR and DP, respectively, and 2.2 min for both of PES and PPA. The LOQ was 10 pg/ml and 50 pg/ml for LOR and PES, respectively. The method is accurate and precise enough for its intended purpose.

Original language	English
Pages (from-to)	217-224
Number of pages	8
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	39
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jpba.2005.03.041
State	Published - 1 Sep 2005
Externally published	Yes

Keywords

LC-MS
Loratadine
Pharmacokinetics
Pseudoephedrine sulfate

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10.1016/j.jpba.2005.03.041

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Sun, J., Wang, G., Wang, W., Zhao, S., Gu, Y., Zhang, J., Huang, M., Shao, F., Li, H., Zhang, Q., & Xie, H. (2005). Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography-electrospray mass spectrometry for pharmacokinetic studies. Journal of Pharmaceutical and Biomedical Analysis, 39(1-2), 217-224. https://doi.org/10.1016/j.jpba.2005.03.041

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title = "Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography-electrospray mass spectrometry for pharmacokinetic studies",

abstract = "To support the pharmacokinetic and bioavailability study of an extended-release loratadine (LOR)/pseudoephedrine sulfate (PES) tablet, a high performance liquid chromatographic-electrospray ionisation-mass spectrometric method (LC-MS) was developed for the simultaneous determination of LOR and PES in human plasma. Diazepam (DP) and phenylpropanolamine (PPA) were used as internal standards for LOR and PES, respectively. Analytes were extracted from alkalized human plasma by liquid/liquid extraction using ethyl ether. Chromatographic separation was performed on an ODS column at flow rate of 0.2 ml/min. The total chromatographic run time was 10.5 min with the retention time of 7.1 min and 6.2 min for LOR and DP, respectively, and 2.2 min for both of PES and PPA. The LOQ was 10 pg/ml and 50 pg/ml for LOR and PES, respectively. The method is accurate and precise enough for its intended purpose.",

keywords = "LC-MS, Loratadine, Pharmacokinetics, Pseudoephedrine sulfate",

author = "Jianguo Sun and Guangji Wang and Wei Wang and Shuai Zhao and Yi Gu and Jinwen Zhang and Mingwen Huang and Fen Shao and Hao Li and Qi Zhang and Haitang Xie",

year = "2005",

month = sep,

day = "1",

doi = "10.1016/j.jpba.2005.03.041",

language = "英语",

volume = "39",

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Sun, J, Wang, G, Wang, W, Zhao, S, Gu, Y, Zhang, J, Huang, M, Shao, F, Li, H, Zhang, Q & Xie, H 2005, 'Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography-electrospray mass spectrometry for pharmacokinetic studies', Journal of Pharmaceutical and Biomedical Analysis, vol. 39, no. 1-2, pp. 217-224. https://doi.org/10.1016/j.jpba.2005.03.041

Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography-electrospray mass spectrometry for pharmacokinetic studies. / Sun, Jianguo; Wang, Guangji; Wang, Wei et al.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 39, No. 1-2, 01.09.2005, p. 217-224.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography-electrospray mass spectrometry for pharmacokinetic studies

AU - Sun, Jianguo

AU - Wang, Guangji

AU - Wang, Wei

AU - Zhao, Shuai

AU - Gu, Yi

AU - Zhang, Jinwen

AU - Huang, Mingwen

AU - Shao, Fen

AU - Li, Hao

AU - Zhang, Qi

AU - Xie, Haitang

PY - 2005/9/1

Y1 - 2005/9/1

N2 - To support the pharmacokinetic and bioavailability study of an extended-release loratadine (LOR)/pseudoephedrine sulfate (PES) tablet, a high performance liquid chromatographic-electrospray ionisation-mass spectrometric method (LC-MS) was developed for the simultaneous determination of LOR and PES in human plasma. Diazepam (DP) and phenylpropanolamine (PPA) were used as internal standards for LOR and PES, respectively. Analytes were extracted from alkalized human plasma by liquid/liquid extraction using ethyl ether. Chromatographic separation was performed on an ODS column at flow rate of 0.2 ml/min. The total chromatographic run time was 10.5 min with the retention time of 7.1 min and 6.2 min for LOR and DP, respectively, and 2.2 min for both of PES and PPA. The LOQ was 10 pg/ml and 50 pg/ml for LOR and PES, respectively. The method is accurate and precise enough for its intended purpose.

AB - To support the pharmacokinetic and bioavailability study of an extended-release loratadine (LOR)/pseudoephedrine sulfate (PES) tablet, a high performance liquid chromatographic-electrospray ionisation-mass spectrometric method (LC-MS) was developed for the simultaneous determination of LOR and PES in human plasma. Diazepam (DP) and phenylpropanolamine (PPA) were used as internal standards for LOR and PES, respectively. Analytes were extracted from alkalized human plasma by liquid/liquid extraction using ethyl ether. Chromatographic separation was performed on an ODS column at flow rate of 0.2 ml/min. The total chromatographic run time was 10.5 min with the retention time of 7.1 min and 6.2 min for LOR and DP, respectively, and 2.2 min for both of PES and PPA. The LOQ was 10 pg/ml and 50 pg/ml for LOR and PES, respectively. The method is accurate and precise enough for its intended purpose.

KW - LC-MS

KW - Loratadine

KW - Pharmacokinetics

KW - Pseudoephedrine sulfate

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SN - 0731-7085

VL - 39

SP - 217

EP - 224

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 1-2

ER -

Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography-electrospray mass spectrometry for pharmacokinetic studies

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