Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis

Tadashi Yoshida; Akira Honda; Naomi Tanaka; Yasushi Matsuzaki; Bingfang He; Toshiaki Osuga; Nobuaki Kobayashi; Kazue Ozawa; Hiroshi Miyazaki

doi:10.1016/0378-4347(93)80133-O

Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis

Tadashi Yoshida, Akira Honda, Naomi Tanaka, Yasushi Matsuzaki, Bingfang He, Toshiaki Osuga, Nobuaki Kobayashi, Kazue Ozawa, Hiroshi Miyazaki

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

A very sensitive and specific method for the simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma is described. The assay is based on isotope dilution mass spectrometry: the extracts from plasma were treated with benzylamine followed by dimethylethylsilylimidazole, then the resulting dimethylethylsilyl ether derivatives of mevalonylbenzylamide and 7α-hydroxycholesterol were determined by gas chromatography-mass spectrometry using high-resolution selected-ion monitoring. Simple regression analysis revealed significant correlations between the plasma level of mevalonate and the hepatic activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34) (r = 0.83, P < 0.01) and between the plasma level of free 7α-hydroxycholesterol and the hepatic activity of cholesterol 7α-hydroxylase (EC 1.14.13.7) (r = 0.76, P < 0.05).

Original language	English
Pages (from-to)	185-193
Number of pages	9
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	613
Issue number	2
DOIs	https://doi.org/10.1016/0378-4347(93)80133-O
State	Published - 2 Apr 1993
Externally published	Yes

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Yoshida, T., Honda, A., Tanaka, N., Matsuzaki, Y., He, B., Osuga, T., Kobayashi, N., Ozawa, K., & Miyazaki, H. (1993). Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis. Journal of Chromatography B: Biomedical Sciences and Applications, 613(2), 185-193. https://doi.org/10.1016/0378-4347(93)80133-O

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title = "Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis",

abstract = "A very sensitive and specific method for the simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma is described. The assay is based on isotope dilution mass spectrometry: the extracts from plasma were treated with benzylamine followed by dimethylethylsilylimidazole, then the resulting dimethylethylsilyl ether derivatives of mevalonylbenzylamide and 7α-hydroxycholesterol were determined by gas chromatography-mass spectrometry using high-resolution selected-ion monitoring. Simple regression analysis revealed significant correlations between the plasma level of mevalonate and the hepatic activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34) (r = 0.83, P < 0.01) and between the plasma level of free 7α-hydroxycholesterol and the hepatic activity of cholesterol 7α-hydroxylase (EC 1.14.13.7) (r = 0.76, P < 0.05).",

author = "Tadashi Yoshida and Akira Honda and Naomi Tanaka and Yasushi Matsuzaki and Bingfang He and Toshiaki Osuga and Nobuaki Kobayashi and Kazue Ozawa and Hiroshi Miyazaki",

year = "1993",

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doi = "10.1016/0378-4347(93)80133-O",

language = "英语",

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Yoshida, T, Honda, A, Tanaka, N, Matsuzaki, Y, He, B, Osuga, T, Kobayashi, N, Ozawa, K & Miyazaki, H 1993, 'Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 613, no. 2, pp. 185-193. https://doi.org/10.1016/0378-4347(93)80133-O

Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis. / Yoshida, Tadashi; Honda, Akira; Tanaka, Naomi et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 613, No. 2, 02.04.1993, p. 185-193.

Research output: Contribution to journal › Article › peer-review

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T1 - Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis

AU - Yoshida, Tadashi

AU - Honda, Akira

AU - Tanaka, Naomi

AU - Matsuzaki, Yasushi

AU - He, Bingfang

AU - Osuga, Toshiaki

AU - Kobayashi, Nobuaki

AU - Ozawa, Kazue

AU - Miyazaki, Hiroshi

PY - 1993/4/2

Y1 - 1993/4/2

N2 - A very sensitive and specific method for the simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma is described. The assay is based on isotope dilution mass spectrometry: the extracts from plasma were treated with benzylamine followed by dimethylethylsilylimidazole, then the resulting dimethylethylsilyl ether derivatives of mevalonylbenzylamide and 7α-hydroxycholesterol were determined by gas chromatography-mass spectrometry using high-resolution selected-ion monitoring. Simple regression analysis revealed significant correlations between the plasma level of mevalonate and the hepatic activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34) (r = 0.83, P < 0.01) and between the plasma level of free 7α-hydroxycholesterol and the hepatic activity of cholesterol 7α-hydroxylase (EC 1.14.13.7) (r = 0.76, P < 0.05).

AB - A very sensitive and specific method for the simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma is described. The assay is based on isotope dilution mass spectrometry: the extracts from plasma were treated with benzylamine followed by dimethylethylsilylimidazole, then the resulting dimethylethylsilyl ether derivatives of mevalonylbenzylamide and 7α-hydroxycholesterol were determined by gas chromatography-mass spectrometry using high-resolution selected-ion monitoring. Simple regression analysis revealed significant correlations between the plasma level of mevalonate and the hepatic activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34) (r = 0.83, P < 0.01) and between the plasma level of free 7α-hydroxycholesterol and the hepatic activity of cholesterol 7α-hydroxylase (EC 1.14.13.7) (r = 0.76, P < 0.05).

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JF - Journal of Chromatography B: Biomedical Sciences and Applications

IS - 2

ER -

Simultaneous determination of mevalonate and 7α-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis

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