Spirolactone and spirothiolactone rhodamine-pyrene probes for detection of Hg2+ with different sensing properties and its application in living cells

Qing Qing Rui; Yi Zhou; Yuan Fang; Cheng Yao

doi:10.1016/j.saa.2016.01.024

Spirolactone and spirothiolactone rhodamine-pyrene probes for detection of Hg²⁺ with different sensing properties and its application in living cells

Qing Qing Rui, Yi Zhou, Yuan Fang, Cheng Yao

School of Chemistry and Moleculai Engineering

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Two new rhodamine B-based fluorescent probes PyRbS and PyRbO containing pyrene moiety were designed and synthesized. Both of the probes showed colorimetric and fluorometric sensing abilities for Hg²⁺ with high selectivity over other metal ions. The binding analysis using Job's plot suggested 1:1 stoichiometry for the complexes formed for Hg²⁺. Compared with PyRbO, the PyRbS showed higher selectivity and sensitivity due to the thiophilic property of Hg²⁺ ion. The PyRbS exhibited the linear fluorescence quenching to Hg²⁺ in the range of 0.3 to 4.8 μM (λ_ex = 365 nm) and 0.3 to 5.4 μM (λ_ex = 515 nm), and the detection limit was 0.72 μM. Moreover, ratiometric changes of PyRbS with Hg²⁺ in absorption spectrum were observed, which could not be obtained in the combination of PyRbO with Hg²⁺. In addition, the methyl thiazolyl tetrazolium (MTT) assay demonstrated that RbPyS had low cytotoxicity and was successfully used to monitor intracellular Hg²⁺ levels in living cells.

Original language	English
Pages (from-to)	209-218
Number of pages	10
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	159
DOIs	https://doi.org/10.1016/j.saa.2016.01.024
State	Published - 15 Apr 2016

Keywords

Fluorescent probe
Hg ion
Live-cell imaging
Rhodamine B

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10.1016/j.saa.2016.01.024

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title = "Spirolactone and spirothiolactone rhodamine-pyrene probes for detection of Hg2+ with different sensing properties and its application in living cells",

abstract = "Two new rhodamine B-based fluorescent probes PyRbS and PyRbO containing pyrene moiety were designed and synthesized. Both of the probes showed colorimetric and fluorometric sensing abilities for Hg2+ with high selectivity over other metal ions. The binding analysis using Job's plot suggested 1:1 stoichiometry for the complexes formed for Hg2+. Compared with PyRbO, the PyRbS showed higher selectivity and sensitivity due to the thiophilic property of Hg2+ ion. The PyRbS exhibited the linear fluorescence quenching to Hg2+ in the range of 0.3 to 4.8 μM (λex = 365 nm) and 0.3 to 5.4 μM (λex = 515 nm), and the detection limit was 0.72 μM. Moreover, ratiometric changes of PyRbS with Hg2+ in absorption spectrum were observed, which could not be obtained in the combination of PyRbO with Hg2+. In addition, the methyl thiazolyl tetrazolium (MTT) assay demonstrated that RbPyS had low cytotoxicity and was successfully used to monitor intracellular Hg2+ levels in living cells.",

keywords = "Fluorescent probe, Hg ion, Live-cell imaging, Rhodamine B",

author = "Rui, {Qing Qing} and Yi Zhou and Yuan Fang and Cheng Yao",

year = "2016",

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day = "15",

doi = "10.1016/j.saa.2016.01.024",

language = "英语",

volume = "159",

pages = "209--218",

journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",

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}

Spirolactone and spirothiolactone rhodamine-pyrene probes for detection of Hg²⁺ with different sensing properties and its application in living cells. / Rui, Qing Qing; Zhou, Yi; Fang, Yuan et al.
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 159, 15.04.2016, p. 209-218.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Spirolactone and spirothiolactone rhodamine-pyrene probes for detection of Hg2+ with different sensing properties and its application in living cells

AU - Rui, Qing Qing

AU - Zhou, Yi

AU - Fang, Yuan

AU - Yao, Cheng

PY - 2016/4/15

Y1 - 2016/4/15

N2 - Two new rhodamine B-based fluorescent probes PyRbS and PyRbO containing pyrene moiety were designed and synthesized. Both of the probes showed colorimetric and fluorometric sensing abilities for Hg2+ with high selectivity over other metal ions. The binding analysis using Job's plot suggested 1:1 stoichiometry for the complexes formed for Hg2+. Compared with PyRbO, the PyRbS showed higher selectivity and sensitivity due to the thiophilic property of Hg2+ ion. The PyRbS exhibited the linear fluorescence quenching to Hg2+ in the range of 0.3 to 4.8 μM (λex = 365 nm) and 0.3 to 5.4 μM (λex = 515 nm), and the detection limit was 0.72 μM. Moreover, ratiometric changes of PyRbS with Hg2+ in absorption spectrum were observed, which could not be obtained in the combination of PyRbO with Hg2+. In addition, the methyl thiazolyl tetrazolium (MTT) assay demonstrated that RbPyS had low cytotoxicity and was successfully used to monitor intracellular Hg2+ levels in living cells.

AB - Two new rhodamine B-based fluorescent probes PyRbS and PyRbO containing pyrene moiety were designed and synthesized. Both of the probes showed colorimetric and fluorometric sensing abilities for Hg2+ with high selectivity over other metal ions. The binding analysis using Job's plot suggested 1:1 stoichiometry for the complexes formed for Hg2+. Compared with PyRbO, the PyRbS showed higher selectivity and sensitivity due to the thiophilic property of Hg2+ ion. The PyRbS exhibited the linear fluorescence quenching to Hg2+ in the range of 0.3 to 4.8 μM (λex = 365 nm) and 0.3 to 5.4 μM (λex = 515 nm), and the detection limit was 0.72 μM. Moreover, ratiometric changes of PyRbS with Hg2+ in absorption spectrum were observed, which could not be obtained in the combination of PyRbO with Hg2+. In addition, the methyl thiazolyl tetrazolium (MTT) assay demonstrated that RbPyS had low cytotoxicity and was successfully used to monitor intracellular Hg2+ levels in living cells.

KW - Fluorescent probe

KW - Hg ion

KW - Live-cell imaging

KW - Rhodamine B

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JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

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ER -

Spirolactone and spirothiolactone rhodamine-pyrene probes for detection of Hg²⁺ with different sensing properties and its application in living cells

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Keywords

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