SpyCatcher/SpyTag-Mediated Self-Assembly of a Supramolecular Complex for Improved Biocatalytic Production of Trehalose

X. Shen; S. Tang; Q. Xu; H. Huang; L. Jiang

doi:10.1134/S0003683819060115

SpyCatcher/SpyTag-Mediated Self-Assembly of a Supramolecular Complex for Improved Biocatalytic Production of Trehalose

X. Shen, S. Tang, Q. Xu, H. Huang, L. Jiang

COLLEGE OF FOOD SCIENCE AND LIGHT INDUSTRY

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Trehalose has been utilized in the food, cosmetic, and pharmaceutical industries due to its ability to stabilize biomolecules. In this study, we developed a strategy of constructing a self-assembling supramolecular multienzyme complex for the production of trehalose using SpyCatcher and SpyTag. Maltooligosyl trehalose synthase (MTS) and maltooligosyl trehalose trehalohydrolase (MTH) were fused to SpyCatcher and its corresponding ligand, SpyTag. The fused MTS-SpyCatcher (MTS-SC) and MTH-SpyTag (MTH-ST) self-assembled into a supramolecular multienzyme complex (MTS-MTH) via the isopeptide bonds formed between SpyCatcher and SpyTag. MTS-MTH showed the faster reaction rate, which reached the peak of trehalose conversion rate 10 h earlier than the free enzymes. The results demonstrate that the multienzyme self-assembly via the protein-peptide interactions of SpyCatcher and SpyTag can increase the catalytic efficiency of sequential reactions.

Original language	English
Pages (from-to)	596-602
Number of pages	7
Journal	Applied Biochemistry and Microbiology
Volume	55
Issue number	6
DOIs	https://doi.org/10.1134/S0003683819060115
State	Published - 1 Nov 2019

Keywords

SpyCatcher
SpyTag
maltooligosyl trehalose synthase
maltooligosyl trehalose trehalohydrolase
multienzyme self-assembly
trehalose

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title = "SpyCatcher/SpyTag-Mediated Self-Assembly of a Supramolecular Complex for Improved Biocatalytic Production of Trehalose",

abstract = "Trehalose has been utilized in the food, cosmetic, and pharmaceutical industries due to its ability to stabilize biomolecules. In this study, we developed a strategy of constructing a self-assembling supramolecular multienzyme complex for the production of trehalose using SpyCatcher and SpyTag. Maltooligosyl trehalose synthase (MTS) and maltooligosyl trehalose trehalohydrolase (MTH) were fused to SpyCatcher and its corresponding ligand, SpyTag. The fused MTS-SpyCatcher (MTS-SC) and MTH-SpyTag (MTH-ST) self-assembled into a supramolecular multienzyme complex (MTS-MTH) via the isopeptide bonds formed between SpyCatcher and SpyTag. MTS-MTH showed the faster reaction rate, which reached the peak of trehalose conversion rate 10 h earlier than the free enzymes. The results demonstrate that the multienzyme self-assembly via the protein-peptide interactions of SpyCatcher and SpyTag can increase the catalytic efficiency of sequential reactions.",

keywords = "SpyCatcher, SpyTag, maltooligosyl trehalose synthase, maltooligosyl trehalose trehalohydrolase, multienzyme self-assembly, trehalose",

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AU - Shen, X.

AU - Tang, S.

AU - Xu, Q.

AU - Huang, H.

AU - Jiang, L.

PY - 2019/11/1

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N2 - Trehalose has been utilized in the food, cosmetic, and pharmaceutical industries due to its ability to stabilize biomolecules. In this study, we developed a strategy of constructing a self-assembling supramolecular multienzyme complex for the production of trehalose using SpyCatcher and SpyTag. Maltooligosyl trehalose synthase (MTS) and maltooligosyl trehalose trehalohydrolase (MTH) were fused to SpyCatcher and its corresponding ligand, SpyTag. The fused MTS-SpyCatcher (MTS-SC) and MTH-SpyTag (MTH-ST) self-assembled into a supramolecular multienzyme complex (MTS-MTH) via the isopeptide bonds formed between SpyCatcher and SpyTag. MTS-MTH showed the faster reaction rate, which reached the peak of trehalose conversion rate 10 h earlier than the free enzymes. The results demonstrate that the multienzyme self-assembly via the protein-peptide interactions of SpyCatcher and SpyTag can increase the catalytic efficiency of sequential reactions.

AB - Trehalose has been utilized in the food, cosmetic, and pharmaceutical industries due to its ability to stabilize biomolecules. In this study, we developed a strategy of constructing a self-assembling supramolecular multienzyme complex for the production of trehalose using SpyCatcher and SpyTag. Maltooligosyl trehalose synthase (MTS) and maltooligosyl trehalose trehalohydrolase (MTH) were fused to SpyCatcher and its corresponding ligand, SpyTag. The fused MTS-SpyCatcher (MTS-SC) and MTH-SpyTag (MTH-ST) self-assembled into a supramolecular multienzyme complex (MTS-MTH) via the isopeptide bonds formed between SpyCatcher and SpyTag. MTS-MTH showed the faster reaction rate, which reached the peak of trehalose conversion rate 10 h earlier than the free enzymes. The results demonstrate that the multienzyme self-assembly via the protein-peptide interactions of SpyCatcher and SpyTag can increase the catalytic efficiency of sequential reactions.

KW - SpyCatcher

KW - SpyTag

KW - maltooligosyl trehalose synthase

KW - maltooligosyl trehalose trehalohydrolase

KW - multienzyme self-assembly

KW - trehalose

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M3 - 文章

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JO - Applied Biochemistry and Microbiology

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ER -

SpyCatcher/SpyTag-Mediated Self-Assembly of a Supramolecular Complex for Improved Biocatalytic Production of Trehalose

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