Tailoring the Oxidative Stress Tolerance of Clostridium tyrobutyricum CCTCC W428 by Introducing Trehalose Biosynthetic Capability

Qian Wu, Liying Zhu, Qing Xu, He Huang, Ling Jiang, Shang Tian Yang

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. This work aims to improve the oxidative stress tolerance of C. tyrobutyricum CCTCC W428, an ideal butyric-acid-producing anaerobe, via the introduction of trehalose biosynthesis capability. Compared with the wild type, the engineered strain showed a wider substrate spectrum, an improved metabolic profile, and a significantly increased specific growth rate upon aeration and acid challenge. Molecular simulation experiments indicated that CoA transferase maintained its native folded state when protected by the trehalose system. Furthermore, qRT-PCR was combined assays for acid-related enzyme activities under various conditions to verify the effects of trehalose. These results demonstrate that introducing a trehalose biosynthetic pathway, which is redundant for the metabolism of C. tyrobutyricum, can increase the robustness of the host to achieve a better oxidative resistance.

Original languageEnglish
Pages (from-to)8892-8901
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number40
DOIs
StatePublished - 11 Oct 2017

Keywords

  • Clostridium tyrobutyricum
  • omics analysis
  • oxidative stress tolerance
  • trehalose synthase

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