Taonella mepensis gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from activated sludge

Xue Dong Xi, Wei Liang Dong, Jun Zhang, Yan Huang, Zhong Li Cui

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9 Scopus citations

Abstract

A novel Gram-negative, non-spore-forming, rod-shaped strain, H1T, was isolated from activated sludge by micromanipulation. No close relatives among cultured bacterial isolates were found; phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H1T forms a deep single branch in the family Rhodospirillaceae. Cells of strain H1T were slightly curved to straight rods (1.2-1.4×1.5-1.7 μm) and motile by a single polar flagellum. Strain H1T was able to grow in the presence of 0-4 % NaCl and grew optimally at 37 °C and pH 6.0-7.0. Chemotaxonomic analysis revealed that strain H1T possessed Q-10 as the predominant ubiquinone and C18: 1ω7c, C16: 0 and C19: 0 cyclo ω8c as the major fatty acids. The DNA G+C content of strain H1T was 65.1 mol%. Comparative analysis of 16S rRNA gene sequences, and phenotypic and chemotaxonomic data, indicate that strain H1T should represent a novel genus and species of the family Rhodospirillaceae. The name Taonella mepensis gen. nov., sp. nov. is proposed. The type strain of Taonella mepensis is H1T (= CICC 10529T = CCTCC AB 2012861T = KACC 16940T).

Original languageEnglish
Pages (from-to)2472-2476
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART7
DOIs
StatePublished - 1 Jul 2013
Externally publishedYes

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