TY - JOUR
T1 - Target-induced reconstruction of Ru(bpy)32+-loaded gold nanocage for one-step highly sensitive detection of Hg2+
AU - Wang, Jin
AU - Lu, Xinyu
AU - Wang, Huafeng
AU - Zhong, Yuan
AU - Dai, Zhihui
AU - Wei, Tianxiang
N1 - Publisher Copyright:
© 2025
PY - 2025/9/1
Y1 - 2025/9/1
N2 - In this work, Ru(bpy)32+-loaded gold nanocage (AuNCs) (Ru–AuNCs) was prepared and found to display a distinct property of electrochemiluminescence (ECL) enhancement under mercury ions (Hg2+) interaction. Based on this, we designed a screen-printed bipolar electrode-ECL (SPBPE-ECL) sensing platform by coupling with the thymine-Hg2+-thymine (T-Hg2+-T) binding pattern for one-step highly sensitive detection of Hg2+. This ECL sensor showed a wide linear detection range (0.75 – 850 μg L−1) and low detection limit (0.1290 μg L−1) toward Hg2+, with a one-step detection procedure and disposable feature, displaying potential applicability in the point-of-care-testing (POCT) of Hg2+ in the environment. In addition, the Hg2+-mediated ECL signal enhancement mechanism of Ru–AuNCs was also investigated. It was confirmed that Hg2+ interaction etched the cage structure of Ru–AuNCs, which sped up the release of more Ru(bpy)32+ around the sensing electrode. Furthermore, Au–Hg alloy structure was formed on the surface of Ru–AuNCs, which also improved the ECL signal. This target-induced in-situ sensing material surface reconstruction strategy would provide a better design concept for the construction of ECL POCT sensor.
AB - In this work, Ru(bpy)32+-loaded gold nanocage (AuNCs) (Ru–AuNCs) was prepared and found to display a distinct property of electrochemiluminescence (ECL) enhancement under mercury ions (Hg2+) interaction. Based on this, we designed a screen-printed bipolar electrode-ECL (SPBPE-ECL) sensing platform by coupling with the thymine-Hg2+-thymine (T-Hg2+-T) binding pattern for one-step highly sensitive detection of Hg2+. This ECL sensor showed a wide linear detection range (0.75 – 850 μg L−1) and low detection limit (0.1290 μg L−1) toward Hg2+, with a one-step detection procedure and disposable feature, displaying potential applicability in the point-of-care-testing (POCT) of Hg2+ in the environment. In addition, the Hg2+-mediated ECL signal enhancement mechanism of Ru–AuNCs was also investigated. It was confirmed that Hg2+ interaction etched the cage structure of Ru–AuNCs, which sped up the release of more Ru(bpy)32+ around the sensing electrode. Furthermore, Au–Hg alloy structure was formed on the surface of Ru–AuNCs, which also improved the ECL signal. This target-induced in-situ sensing material surface reconstruction strategy would provide a better design concept for the construction of ECL POCT sensor.
KW - Au–Hg alloy
KW - Electrochemiluminescence biosensing
KW - Mercury ions
KW - Point-of-care testing
KW - Ru(bpy)-loaded gold nanocages
KW - Screen-printed bipolar electrode
UR - http://www.scopus.com/inward/record.url?scp=105000178566&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2025.127955
DO - 10.1016/j.talanta.2025.127955
M3 - 文章
AN - SCOPUS:105000178566
SN - 0039-9140
VL - 292
JO - Talanta
JF - Talanta
M1 - 127955
ER -