Temperature-driven CsgA-enhanced mutant enable distinct self-assembly

Functional coating materials have found extensive applications across various technological fields. However, the effectiveness of these coating depends critically on the choice of an appropriate medium. In this study, we developed an advanced “molecular glue”, a CsgA variant known as CsgA-pro, which can serve as a versatile medium for biotherapy. CsgA-pro exhibits a remarkable temperature-driven transition, forming millimeter-scale protein fibers at low temperatures and transitioning to a “molecular glue” state at elevated temperatures. To demonstrate its potential as a “molecular glue”, CsgA-pro-GFP was first immobilized onto a gauze surface, which was subsequently treated with chloroauric acid to generate gauze loaded with gold nanoparticles (Au NPs). These Au NPs were shown to possess potent antibacterial properties and to promote migration of L929 and HUVEC cells. When applied to wound healing, the Au NP-loaded gauze significantly accelerated the healing process compared to control groups, highlighting the therapeutic potential of this approach. These findings underscore the promise of CsgA-pro as a multifunctional tool in a wide range of applications, including multi-enzyme biocatalysis and immobilization. We envision that CsgA-pro will have broad applicability across diverse fields, offering substantial contributions to both technological innovations and biomedical advancements.