The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5′-monophosphate by Saccharomyces cerevisiae

Yong Chen; Shuya Li; Jian Xiong; Zhenjiang Li; Jianxin Bai; Lei Zhang; Qi Ye; Pingkai Ouyang; Hanjie Ying

doi:10.1007/s00253-009-2287-y

The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5′-monophosphate by Saccharomyces cerevisiae

Yong Chen, Shuya Li, Jian Xiong, Zhenjiang Li, Jianxin Bai, Lei Zhang, Qi Ye, Pingkai Ouyang, Hanjie Ying

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A whole cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 gl^-1 sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.

Original language	English
Pages (from-to)	75-81
Number of pages	7
Journal	Applied Microbiology and Biotechnology
Volume	86
Issue number	1
DOIs	https://doi.org/10.1007/s00253-009-2287-y
State	Published - Mar 2010

Keywords

Carbon flux
Citrate
Saccharomyces cerevisiae
Uridine 5′-monophosphate

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abstract = "A whole cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 gl-1 sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.",

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author = "Yong Chen and Shuya Li and Jian Xiong and Zhenjiang Li and Jianxin Bai and Lei Zhang and Qi Ye and Pingkai Ouyang and Hanjie Ying",

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AU - Chen, Yong

AU - Li, Shuya

AU - Xiong, Jian

AU - Li, Zhenjiang

AU - Bai, Jianxin

AU - Zhang, Lei

AU - Ye, Qi

AU - Ouyang, Pingkai

AU - Ying, Hanjie

PY - 2010/3

Y1 - 2010/3

N2 - A whole cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 gl-1 sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.

AB - A whole cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 gl-1 sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.

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KW - Citrate

KW - Saccharomyces cerevisiae

KW - Uridine 5′-monophosphate

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The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5′-monophosphate by Saccharomyces cerevisiae

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Keywords

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