Abstract
The effect of plasmolysing agents or detergents to cell permeability and enzymatic activity and the technological conditions of synthesizing fructose-1,6-diphosphate were studied. It was separated with anion ion exchange resign and crystallized in ethanoladdition system according to the differences of physical-chemical characters of all intraccllular intermediates. The yield and purity of product are 75%∼80% and 99.5%, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 280-281 |
| Number of pages | 2 |
| Journal | Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology |
| Volume | 14 |
| Issue number | 3 |
| State | Published - 1998 |
Keywords
- Fructose-1,6-diphosphate
- Preparation and purification