TY - JOUR
T1 - Transcriptome analysis of Clostridium beijerinckii adaptation mechanisms in response to ferulic acid
AU - Liu, Jun
AU - Guo, Ting
AU - Yang, Tao
AU - Xu, Jiahui
AU - Tang, Chenglun
AU - Liu, Dong
AU - Ying, Hanjie
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/5/1
Y1 - 2017/5/1
N2 - Clostridium beijerinckii 4693:int with high ferulic acid (FA) tolerance was engineered and characterized in our lab. In this study, the minimum inhibition concentrations of FA against C. beijerinckii NCIMB 8052 (wild-type) and 4693:int were 1.0 and 1.5 g/l, respectively; cell viability was 18.5% and 106.7%, respectively, in the presence of 0.5 g/l FA. A comparative transcriptome analysis was carried out at two different growth stages to evaluate sensitivity to FA. Genes that were differentially expressed included those related to redox and associated cofactors, riboflavin metabolism, two-component system, glycolysis and butanoate metabolism, and DNA replication as well as those encoding ATP-binding cassette transporters. Cbei_2134 and Cbei_2135 encoding alkyl hydroperoxide reductases are thought to be involved in antibacterial and adaptation mechanisms in C. beijerinckii in the presence of FA.
AB - Clostridium beijerinckii 4693:int with high ferulic acid (FA) tolerance was engineered and characterized in our lab. In this study, the minimum inhibition concentrations of FA against C. beijerinckii NCIMB 8052 (wild-type) and 4693:int were 1.0 and 1.5 g/l, respectively; cell viability was 18.5% and 106.7%, respectively, in the presence of 0.5 g/l FA. A comparative transcriptome analysis was carried out at two different growth stages to evaluate sensitivity to FA. Genes that were differentially expressed included those related to redox and associated cofactors, riboflavin metabolism, two-component system, glycolysis and butanoate metabolism, and DNA replication as well as those encoding ATP-binding cassette transporters. Cbei_2134 and Cbei_2135 encoding alkyl hydroperoxide reductases are thought to be involved in antibacterial and adaptation mechanisms in C. beijerinckii in the presence of FA.
KW - Clostridium beijerinckii
KW - Comparative transcriptome analysis
KW - Ferulic acid tolerance
KW - Metabolism pathway
UR - http://www.scopus.com/inward/record.url?scp=85014861121&partnerID=8YFLogxK
U2 - 10.1016/j.biocel.2017.02.009
DO - 10.1016/j.biocel.2017.02.009
M3 - 文章
C2 - 28242360
AN - SCOPUS:85014861121
SN - 1357-2725
VL - 86
SP - 14
EP - 21
JO - International Journal of Biochemistry and Cell Biology
JF - International Journal of Biochemistry and Cell Biology
ER -