Transglycosylation of stevioside to improve the edulcorant quality by lower substitution using cornstarch hydrolyzate and CGTase

Sha Li; Wei Li; Qiu Yan Xiao; Yongmei Xia

doi:10.1016/j.foodchem.2012.10.124

Transglycosylation of stevioside to improve the edulcorant quality by lower substitution using cornstarch hydrolyzate and CGTase

Sha Li, Wei Li, Qiu Yan Xiao, Yongmei Xia

COLLEGE OF FOOD SCIENCE AND LIGHT INDUSTRY

Jiangnan University

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Stevioside is an abundant sweetener extracted from Stevia rebaudiana leaf with a bitter aftertaste. Enzymatic transglycosylation of stevioside is a solution to improve the edulcorant quality of stevioside, but highly derivatised stevioside coming with high conversion of stevioside is undesired. In this experiment, the transglycosylation of stevioside was investigated by using a commercial cyclodextrin glucanotransferase and cornstarch hydrolyzate. With controlled parameters, the product was mainly composed of mono- and di-glucosylated stevioside while the highest stevioside conversion reached 77.11%. Neither kinetic nor thermodynamic factor stimulated the formation of high substituted steviosides. The simultaneous hydrolysis in the reaction might inhibit the yield of highly substituted steviosides.

Original language	English
Pages (from-to)	2064-2069
Number of pages	6
Journal	Food Chemistry
Volume	138
Issue number	2-3
DOIs	https://doi.org/10.1016/j.foodchem.2012.10.124
State	Published - 1 Jun 2013

Keywords

Cyclodextrin glucanotransferase
Enzymatic
Stevioside
Transglycosylation

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10.1016/j.foodchem.2012.10.124

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title = "Transglycosylation of stevioside to improve the edulcorant quality by lower substitution using cornstarch hydrolyzate and CGTase",

abstract = "Stevioside is an abundant sweetener extracted from Stevia rebaudiana leaf with a bitter aftertaste. Enzymatic transglycosylation of stevioside is a solution to improve the edulcorant quality of stevioside, but highly derivatised stevioside coming with high conversion of stevioside is undesired. In this experiment, the transglycosylation of stevioside was investigated by using a commercial cyclodextrin glucanotransferase and cornstarch hydrolyzate. With controlled parameters, the product was mainly composed of mono- and di-glucosylated stevioside while the highest stevioside conversion reached 77.11%. Neither kinetic nor thermodynamic factor stimulated the formation of high substituted steviosides. The simultaneous hydrolysis in the reaction might inhibit the yield of highly substituted steviosides.",

keywords = "Cyclodextrin glucanotransferase, Enzymatic, Stevioside, Transglycosylation",

author = "Sha Li and Wei Li and Xiao, {Qiu Yan} and Yongmei Xia",

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AU - Li, Sha

AU - Li, Wei

AU - Xiao, Qiu Yan

AU - Xia, Yongmei

PY - 2013/6/1

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AB - Stevioside is an abundant sweetener extracted from Stevia rebaudiana leaf with a bitter aftertaste. Enzymatic transglycosylation of stevioside is a solution to improve the edulcorant quality of stevioside, but highly derivatised stevioside coming with high conversion of stevioside is undesired. In this experiment, the transglycosylation of stevioside was investigated by using a commercial cyclodextrin glucanotransferase and cornstarch hydrolyzate. With controlled parameters, the product was mainly composed of mono- and di-glucosylated stevioside while the highest stevioside conversion reached 77.11%. Neither kinetic nor thermodynamic factor stimulated the formation of high substituted steviosides. The simultaneous hydrolysis in the reaction might inhibit the yield of highly substituted steviosides.

KW - Cyclodextrin glucanotransferase

KW - Enzymatic

KW - Stevioside

KW - Transglycosylation

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Transglycosylation of stevioside to improve the edulcorant quality by lower substitution using cornstarch hydrolyzate and CGTase

Abstract

Keywords

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