Ultrasensitive electrochemical biosensing for DNA using quantum dots combined with restriction endonuclease

Can Zhang; Jing Lou; Wenwen Tu; Jianchun Bao; Zhihui Dai

doi:10.1039/c4an01284d

Ultrasensitive electrochemical biosensing for DNA using quantum dots combined with restriction endonuclease

Can Zhang, Jing Lou, Wenwen Tu, Jianchun Bao, Zhihui Dai

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

A universal and sensitive electrochemical biosensing platform for the detection and identification of DNA using CdSe quantum dots (CdSe QDs) as signal markers was designed. The detection mechanism was based on the specific recognition of MspI endonuclease combined with the signal amplification of gold nanoparticles (AuNPs). MspI endonuclease could recognize its specific sequence in the double-strand DNA (dsDNA) and cleave the dsDNA fragments linked with CdSe QDs from the electrode. The remaining attached CdSe QDs can be easily read out by square-wave voltammetry using an electrodeposited bismuth (Bi) film-modified glass carbon electrode. The concentrations of target DNA could be simultaneously detected by the signal of metal markers. Using mycobacterium tuberculosis (Mtb) DNA as a model, under the optimal conditions, the proposed biosensor could detect Mtb DNA down to 8.7 × 10^-15 M with a linear range of 5 orders of magnitude (from 1.0 × 10^-14 to 1.0 × 10^-9 M) and discriminate mismatched DNA with high selectivity. This strategy presented a universal and convenient biosensing platform for DNA assay, and its satisfactory performances make it a potential candidate for the early diagnosis of gene-related diseases.

Original language	English
Pages (from-to)	506-511
Number of pages	6
Journal	The Analyst
Volume	140
Issue number	2
DOIs	https://doi.org/10.1039/c4an01284d
State	Published - 21 Jan 2015
Externally published	Yes

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title = "Ultrasensitive electrochemical biosensing for DNA using quantum dots combined with restriction endonuclease",

abstract = "A universal and sensitive electrochemical biosensing platform for the detection and identification of DNA using CdSe quantum dots (CdSe QDs) as signal markers was designed. The detection mechanism was based on the specific recognition of MspI endonuclease combined with the signal amplification of gold nanoparticles (AuNPs). MspI endonuclease could recognize its specific sequence in the double-strand DNA (dsDNA) and cleave the dsDNA fragments linked with CdSe QDs from the electrode. The remaining attached CdSe QDs can be easily read out by square-wave voltammetry using an electrodeposited bismuth (Bi) film-modified glass carbon electrode. The concentrations of target DNA could be simultaneously detected by the signal of metal markers. Using mycobacterium tuberculosis (Mtb) DNA as a model, under the optimal conditions, the proposed biosensor could detect Mtb DNA down to 8.7 × 10-15 M with a linear range of 5 orders of magnitude (from 1.0 × 10-14 to 1.0 × 10-9 M) and discriminate mismatched DNA with high selectivity. This strategy presented a universal and convenient biosensing platform for DNA assay, and its satisfactory performances make it a potential candidate for the early diagnosis of gene-related diseases.",

author = "Can Zhang and Jing Lou and Wenwen Tu and Jianchun Bao and Zhihui Dai",

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T1 - Ultrasensitive electrochemical biosensing for DNA using quantum dots combined with restriction endonuclease

AU - Zhang, Can

AU - Lou, Jing

AU - Tu, Wenwen

AU - Bao, Jianchun

AU - Dai, Zhihui

PY - 2015/1/21

Y1 - 2015/1/21

N2 - A universal and sensitive electrochemical biosensing platform for the detection and identification of DNA using CdSe quantum dots (CdSe QDs) as signal markers was designed. The detection mechanism was based on the specific recognition of MspI endonuclease combined with the signal amplification of gold nanoparticles (AuNPs). MspI endonuclease could recognize its specific sequence in the double-strand DNA (dsDNA) and cleave the dsDNA fragments linked with CdSe QDs from the electrode. The remaining attached CdSe QDs can be easily read out by square-wave voltammetry using an electrodeposited bismuth (Bi) film-modified glass carbon electrode. The concentrations of target DNA could be simultaneously detected by the signal of metal markers. Using mycobacterium tuberculosis (Mtb) DNA as a model, under the optimal conditions, the proposed biosensor could detect Mtb DNA down to 8.7 × 10-15 M with a linear range of 5 orders of magnitude (from 1.0 × 10-14 to 1.0 × 10-9 M) and discriminate mismatched DNA with high selectivity. This strategy presented a universal and convenient biosensing platform for DNA assay, and its satisfactory performances make it a potential candidate for the early diagnosis of gene-related diseases.

AB - A universal and sensitive electrochemical biosensing platform for the detection and identification of DNA using CdSe quantum dots (CdSe QDs) as signal markers was designed. The detection mechanism was based on the specific recognition of MspI endonuclease combined with the signal amplification of gold nanoparticles (AuNPs). MspI endonuclease could recognize its specific sequence in the double-strand DNA (dsDNA) and cleave the dsDNA fragments linked with CdSe QDs from the electrode. The remaining attached CdSe QDs can be easily read out by square-wave voltammetry using an electrodeposited bismuth (Bi) film-modified glass carbon electrode. The concentrations of target DNA could be simultaneously detected by the signal of metal markers. Using mycobacterium tuberculosis (Mtb) DNA as a model, under the optimal conditions, the proposed biosensor could detect Mtb DNA down to 8.7 × 10-15 M with a linear range of 5 orders of magnitude (from 1.0 × 10-14 to 1.0 × 10-9 M) and discriminate mismatched DNA with high selectivity. This strategy presented a universal and convenient biosensing platform for DNA assay, and its satisfactory performances make it a potential candidate for the early diagnosis of gene-related diseases.

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Ultrasensitive electrochemical biosensing for DNA using quantum dots combined with restriction endonuclease

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