合成生物学改造酵母驱动丁二酸绿色生物制造

Succinic acid is an important C₄ platform chemical, and its industrial biomanufacturing route has significant advantages, which is a research hotspot. The microorganisms for succinic acid biosynthesis mainly include bacteria and yeasts. Compared with bacteria, although the product yield of yeasts is lower, the yeasts generally have high acid tolerance, therefore, they can synthesize succinic acid under low pH conditions, thus avoiding adding additional neutralization agent to maintain the neutral pH conditions, therefore, the downstream process for yeast-based succinic acid purification is simpler and costs lower. This article reviews the research progress in the use of yeast microorganisms to synthesize succinic acid in recent years, focusing on the synthetic biology transformation strategies of yeast production strains represented by Saccharomyces cerevisiae, Yarrowia lipolytica, and Issatchenkia orientalis. Finally, the future directions for green biomanufacturing of succinic acid by constructing yeast cell factories are proposed.