吸附树脂对唾液酸的分离纯化

A new adsorbent resin RH-1 was used to recover SA from sialic acid (SA) biotransformation solution. The adsorption equilibrium, adsorption kinetic and dynamic adsorption and desorption processes of SA onto the resin were investigated. The adsorption isotherms of SA at different temperatures were determined. It was observed that the adsorption capacity of SA onto the resin decreased with increase of temperature and the adsorption process was exothermic. The adsorption equilibrium could be described by the Henry adsorption model (R²=0.998). The surface diffusion model could better describe the kinetic behaviour of SA adsorption onto the resin. The fitted surface diffusion coefficient D_e was 2.19×10^-9m²/s, and SA could reach adsorption equilibrium within 10min. Finally, the SA in the conversion solution was recovered by column dynamic method. The breakthrough curves of SA on the resin under different operating conditions were investigated. The yield of SA obtained was ≥99.8% with water as the eluent. In this paper, the separation and recovery of SA can be achieved with water, which provides a new method for the green production of SA.