桑木耳多糖提取工艺优化及其益生活性和抗氧化活性评价

The extraction process of of Mulberry fungus polysaccharide (MFP) sampled from Kunming, Yunnan Province, were optimized and the probiotic and antioxidant activities of the extracted polysaccharides were analyzed in this study. By single factor test combined with response surface methodology, the optimized water extraction conditions of MFP were obtained. When the extraction was performed with the solid-liquid ratio of 1:108 (m/V) at 100℃ for 3.5 h, the polysaccharide yield was 6.96%. After 10 repeated extractions, the polysaccharide yield was as high as 34.18%. High performance gel permeation chromatography (HPGPC) analysis showed the average molecular weight of MFP was 2.09×10⁷ u. The monosaccharide composition analyzed by ion chromatography showed that MFP mainly contained glucose, xylose and mannose, their molar mass ratio was 1.18: 0.65: 2.88. Fourier transform infrared (FT-IR) results showed that the polysaccharide contained β-glucopyranoside bonds. The experimental data of the effects of MFP on probiotics indicated that MFP had prebiotic activity that could effectively promote the proliferation of Bifidobacterium bifidum and Bifidobacterium adolescentis. Furthermore, the in vitro antioxidant activity experiment showed that MFP at the concentration of 10 mg/mL had strong ABTS·⁺ and ·OH radical scavenging ability and the scavenging rates were 98.93% and 71.28%, respectively.