添加剂对牛粪堆肥不同阶段真菌群落演替的影响

In order to investigate the effect of physical additives on the fungal community structure during the composting of cattle manure, a 30-day composting experiment was carried out with cattle manure and wheat straw as raw materials, and biochar and volcanic rocks as additives. The requirements of harmless and stablilization of cattle manure composting were achieved in experiments. High-throughput sequencing results showed that Wallemia was dominant in composting raw materials, with a relative abundance of 86.85%. In the thermophilic phase, Wallemia and Aspergillus were the dominant genus in the control treatment and volcanic rocks treatment, while the relative abundance of Scedosporium and Acremonium was higher in the biochar treatment. Mycothermus and Thermomyces were dominant in the cooling phase, and the relative abundance of Thermomyces in the biochar treatment was higher than that in the other two treatments. In the maturity phase, the relative abundance of Mycothermus was the highest among all the treatments. The addition of volcanic rocks increased the abundance of Remersonia and Trichosporon. Spearman correlation analysis showed that Mycothermus was significantly correlated with GI, TN, TP, NH₄ ⁺-N, moisture content, pH, VS and urease (P<0.05). This study provides references for the research on mechanism and process modification of cattle manure composting.