TY - JOUR
T1 - A highly sensitive and reusable electrochemical mercury biosensor based on tunable vertical single-walled carbon nanotubes and a target recycling strategy
AU - Shi, Lei
AU - Wang, Yan
AU - Chu, Zhenyu
AU - Yin, Yu
AU - Jiang, Danfeng
AU - Luo, Jingyi
AU - Ding, Shiming
AU - Jin, Wanqin
N1 - Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2017
Y1 - 2017
N2 - In this work, a novel electrochemical Hg2+ biosensor with high sensitivity and excellent reusability was presented. The sensor was based on tunable vertical single-walled carbon nanotubes (v-SWCNTs) and a target recycling strategy. A facile and scalable approach involving the conformational regulation of self-assembled monolayers was established for the fabrication of v-SWCNTs with tailored orientation and homogeneity. The obtained v-SWCNTs exhibited superior properties including a large specific area, high electrical conductivity, and excellent substrate binding strength, opening up a wide horizon for advanced electrochemical applications. Meanwhile, an efficient Hg2+ recycling strategy was designed using exonuclease III. In this strategy, a trace amount of Hg2+ triggered consecutive nicking reactions, and numerous report probes were released to bind with v-SWCNTs through π-π interactions. Based on the innovative design, an ultralow detection limit of 3 fM (S/N = 3), a wide linear range from 10 fM to 1 μM, high selectivity, and good reliability were achieved for a Hg2+ assay in water and serum samples using the prepared biosensor. Besides, due to the reversibility of π-π interactions, the stable v-SWCNT interface was regenerated for 50 consecutive measurements without obvious signal loss, making it a promising candidate for routine and efficient Hg2+ monitoring.
AB - In this work, a novel electrochemical Hg2+ biosensor with high sensitivity and excellent reusability was presented. The sensor was based on tunable vertical single-walled carbon nanotubes (v-SWCNTs) and a target recycling strategy. A facile and scalable approach involving the conformational regulation of self-assembled monolayers was established for the fabrication of v-SWCNTs with tailored orientation and homogeneity. The obtained v-SWCNTs exhibited superior properties including a large specific area, high electrical conductivity, and excellent substrate binding strength, opening up a wide horizon for advanced electrochemical applications. Meanwhile, an efficient Hg2+ recycling strategy was designed using exonuclease III. In this strategy, a trace amount of Hg2+ triggered consecutive nicking reactions, and numerous report probes were released to bind with v-SWCNTs through π-π interactions. Based on the innovative design, an ultralow detection limit of 3 fM (S/N = 3), a wide linear range from 10 fM to 1 μM, high selectivity, and good reliability were achieved for a Hg2+ assay in water and serum samples using the prepared biosensor. Besides, due to the reversibility of π-π interactions, the stable v-SWCNT interface was regenerated for 50 consecutive measurements without obvious signal loss, making it a promising candidate for routine and efficient Hg2+ monitoring.
UR - http://www.scopus.com/inward/record.url?scp=85011371564&partnerID=8YFLogxK
U2 - 10.1039/C6TB02658C
DO - 10.1039/C6TB02658C
M3 - 文章
C2 - 32263885
AN - SCOPUS:85011371564
SN - 2050-750X
VL - 5
SP - 1073
EP - 1080
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 5
ER -