A highly specific fluorescent probe for hypochlorite based on fluorescein derivative and its endogenous imaging in living cells

Bihui Wang, Dan Chen, Srinivasulu Kambam, Fang Wang, Yong Wang, Wei Zhang, Jun Yin, Haiyan Chen, Xiaoqiang Chen

科研成果: 期刊稿件文章同行评审

94 引用 (Scopus)

摘要

A new fluorescein-based probe is found to be sensitive and selective for the detection of hypochlorite anion in aqueous buffer solution. The probe displays high water solubility, pH-independent fluorescence, and biocompatibility. A possible detection mechanism is that HOCl oxidizes the CN bond into aldehyde, resulting in bright emission. Results show that the probe has a 7.3 nM detection limit to hypochlorite anion. In addition, the probe is successfully utilized for monitoring endogenously produced hypochlorite in living macrophage cells upon stimulation.

源语言英语
页(从-至)22-29
页数8
期刊Dyes and Pigments
120
DOI
出版状态已出版 - 1 9月 2015

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