@article{06979cb9d9c44d48941864456a82945e,
title = "A novel protocol to accelerate dynamic combinatorial chemistry via isolation of ligand-target adducts from dynamic combinatorial libraries: A case study identifying competitive inhibitors of lysozyme",
abstract = "A novel protocol based on size-exclusion chromatography (SEC) and MS was established to accelerate dynamic combinatorial chemistry (DCC) in this study. By isolating ligand-target adducts from the dynamic combinatorial library (DCL), ligands could be identified directly by MS after denaturation. Three new inhibitors for lysozyme were discovered by this SEC-MS protocol in a case study. Km Data for these new inhibitors was also determined.",
keywords = "Combinatorial chemistry, Drug discovery, Enzyme inhibitors, Ligand separation",
author = "Zheng Fang and Wei He and Xin Li and Zhengjiang Li and Beining Chen and Pingkai Ouyang and Kai Guo",
year = "2013",
month = sep,
day = "15",
doi = "10.1016/j.bmcl.2013.07.011",
language = "英语",
volume = "23",
pages = "5174--5177",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Ltd",
number = "18",
}