A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides

Ke Liu; Youzhi Zhou; Jinsong Zhang; Jianlin Chu; Yewei Zhang; Bingfang He

doi:10.1007/s10529-017-2423-1

A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides

Ke Liu, Youzhi Zhou, Jinsong Zhang, Jianlin Chu, Yewei Zhang, Bingfang He

药学院

科研成果: 期刊稿件 › 文章 › 同行评审

3 引用（Scopus）

摘要

Objective: To isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining. Results: BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2′-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 °C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61–84%. Conclusion: An efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria.

源语言	英语
页（从-至）	1903-1910
页数	8
期刊	Biotechnology Letters
卷	39
期	12
DOI	https://doi.org/10.1007/s10529-017-2423-1
出版状态	已出版 - 1 12月 2017

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title = "A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides",

abstract = "Objective: To isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining. Results: BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2′-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 °C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61–84%. Conclusion: An efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria.",

keywords = "Gene mining, Halogenated nucleoside, Pyrimidine nucleoside phosphorylase",

author = "Ke Liu and Youzhi Zhou and Jinsong Zhang and Jianlin Chu and Yewei Zhang and Bingfang He",

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T1 - A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides

AU - Liu, Ke

AU - Zhou, Youzhi

AU - Zhang, Jinsong

AU - Chu, Jianlin

AU - Zhang, Yewei

AU - He, Bingfang

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Objective: To isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining. Results: BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2′-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 °C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61–84%. Conclusion: An efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria.

AB - Objective: To isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining. Results: BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2′-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 °C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61–84%. Conclusion: An efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria.

KW - Gene mining

KW - Halogenated nucleoside

KW - Pyrimidine nucleoside phosphorylase

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SN - 0141-5492

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A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides

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