Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis

Rui Zhang; Xun Cao; Hanxiao Ying; Zemeng Liu; Kequan Chen

doi:10.3969/j.issn.1673-1689.2018.03.006

Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis

Rui Zhang, Xun Cao, Hanxiao Ying, Zemeng Liu, Kequan Chen

生物与制药工程学院

Nanjing Tech University

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

Pipecolic acid is an important precursor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate, a PDMS microchip was designed and fabricated. Effects of microchannel size, microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were: microchannel size 200 |xm; microchannel length 124.8 cm;and liquid flow rate 100 μm/min. Under the optimized conditions, a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction, the reaction time reduced 1/3 in comparison with the reaction in shaking tube.

源语言	英语
页（从-至）	262-267
页数	6
期刊	Journal of Food Science and Biotechnology
卷	37
期	3
DOI	https://doi.org/10.3969/j.issn.1673-1689.2018.03.006
出版状态	已出版 - 2018

访问文件

10.3969/j.issn.1673-1689.2018.03.006

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{b212e0cd3ff7438d80883306d76584fe,

title = "Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis",

abstract = "Pipecolic acid is an important precursor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate, a PDMS microchip was designed and fabricated. Effects of microchannel size, microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were: microchannel size 200 |xm; microchannel length 124.8 cm;and liquid flow rate 100 μm/min. Under the optimized conditions, a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction, the reaction time reduced 1/3 in comparison with the reaction in shaking tube.",

keywords = "Enzyme catalytic, Lysine, Lysine cyclodeaminase, PDMS microchip, Pipecolic acid",

author = "Rui Zhang and Xun Cao and Hanxiao Ying and Zemeng Liu and Kequan Chen",

year = "2018",

doi = "10.3969/j.issn.1673-1689.2018.03.006",

language = "英语",

volume = "37",

pages = "262--267",

journal = "Journal of Food Science and Biotechnology",

issn = "1673-1689",

publisher = "Editorial office of Journal of Food Science and Biotechnology",

number = "3",

}

TY - JOUR

T1 - Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis

AU - Zhang, Rui

AU - Cao, Xun

AU - Ying, Hanxiao

AU - Liu, Zemeng

AU - Chen, Kequan

PY - 2018

Y1 - 2018

N2 - Pipecolic acid is an important precursor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate, a PDMS microchip was designed and fabricated. Effects of microchannel size, microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were: microchannel size 200 |xm; microchannel length 124.8 cm;and liquid flow rate 100 μm/min. Under the optimized conditions, a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction, the reaction time reduced 1/3 in comparison with the reaction in shaking tube.

AB - Pipecolic acid is an important precursor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate, a PDMS microchip was designed and fabricated. Effects of microchannel size, microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were: microchannel size 200 |xm; microchannel length 124.8 cm;and liquid flow rate 100 μm/min. Under the optimized conditions, a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction, the reaction time reduced 1/3 in comparison with the reaction in shaking tube.

KW - Enzyme catalytic

KW - Lysine

KW - Lysine cyclodeaminase

KW - PDMS microchip

KW - Pipecolic acid

UR - http://www.scopus.com/inward/record.url?scp=85048268728&partnerID=8YFLogxK

U2 - 10.3969/j.issn.1673-1689.2018.03.006

DO - 10.3969/j.issn.1673-1689.2018.03.006

M3 - 文章

AN - SCOPUS:85048268728

SN - 1673-1689

VL - 37

SP - 262

EP - 267

JO - Journal of Food Science and Biotechnology

JF - Journal of Food Science and Biotechnology

IS - 3

ER -

Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis

摘要

访问文件

其它文件与链接

指纹

引用此