TY - JOUR
T1 - Cell morphology variations of Klebsiella pneumoniae induced by acetate stress using biomimetic vesicle assay
AU - Lu, Shengguo
AU - Han, Yuwang
AU - Duan, Xujia
AU - Luo, Fang
AU - Zhu, Lingyan
AU - Li, Shuang
AU - Huang, He
PY - 2013/10
Y1 - 2013/10
N2 - Supplementation with acetate under low levels was used as a novel approach to control the morphological development of Klebsiella pneumoniae aimed to improve 1,3-propanediol (1,3-PD) production. A full range of morphological types formed from rod shape to oval shape even round shape in response to different concentrations of acetate. The cell growth and 1,3-PD productions in the shake flasks with 0.5 g/L acetate addition were improved by 9.4 and 28.37 %, respectively, as compared to the control, while the cell became shorter and began to lose its original shape. The cell membrane penetration by acetate was investigated by the biomimetic vesicles, while higher concentration of acetate led to more moderate colorimetric transitions. Moreover, the percentage composition of unsaturated fatty acid (UFA) was increased as well as the increased concentrations of acetate, whereas higher UFA percentage, higher fluidity of bacterial cell membrane.
AB - Supplementation with acetate under low levels was used as a novel approach to control the morphological development of Klebsiella pneumoniae aimed to improve 1,3-propanediol (1,3-PD) production. A full range of morphological types formed from rod shape to oval shape even round shape in response to different concentrations of acetate. The cell growth and 1,3-PD productions in the shake flasks with 0.5 g/L acetate addition were improved by 9.4 and 28.37 %, respectively, as compared to the control, while the cell became shorter and began to lose its original shape. The cell membrane penetration by acetate was investigated by the biomimetic vesicles, while higher concentration of acetate led to more moderate colorimetric transitions. Moreover, the percentage composition of unsaturated fatty acid (UFA) was increased as well as the increased concentrations of acetate, whereas higher UFA percentage, higher fluidity of bacterial cell membrane.
KW - 1,3-Propanediol
KW - Acetate stress
KW - Biomimetic vesicle assay
KW - Cell morphology
KW - Klebsiella pneumoniae
KW - Membrane phospholipids analysis
UR - http://www.scopus.com/inward/record.url?scp=84885184237&partnerID=8YFLogxK
U2 - 10.1007/s12010-013-0368-y
DO - 10.1007/s12010-013-0368-y
M3 - 文章
C2 - 23892619
AN - SCOPUS:84885184237
SN - 0273-2289
VL - 171
SP - 731
EP - 743
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 3
ER -