摘要
The investigation of subtle change of cells exposed to nanomaterials is extremely essential but also challenging for nanomaterial-based biological applications. In this study, atomic force microscopy (AFM) was employed to investigate the effects of iron-iron oxide core-shell magnetic nanoparticles on the mechanical properties of bovine articular chondrocytes (BACs). After being exposed to the nanoparticles even at a high nanoparticle-concentration (50 μg mL-1), no obvious difference was observed by using conventional methods, including the WST-1 assay and live/dead staining. However a significant difference of Young's modulus of the cells was detected by AFM even when the concentration of nanoparticles applied in the cell culture medium was low (10 μg mL-1). The difference of cellular Young's modulus increased with the increase of nanoparticle concentration. AFM was demonstrated to be a useful tool to identify the subtle change of cells when they were exposed to nanomaterials even at a low concentration.
| 源语言 | 英语 |
|---|---|
| 页（从-至） | 1284-1290 |
| 页数 | 7 |
| 期刊 | Biomaterials Science |
| 卷 | 3 |
| 期 | 9 |
| DOI | |
| 出版状态 | 已出版 - 1 9月 2015 |
| 已对外发布 | 是 |