Direct electrochemistry of glucose oxidase immobilized on mesoporous TiO₂

A novel mesoporous TiO₂ material (m-TiO₂) was obtained by a soft chemistry method in the absence of any surfactants or templates. It was shown that the m-TiO₂ had an anatase crystalline structure with well-distributed mesopores from XRD, N₂ adsorption-desorption isotherms, FESEM and TEM. The FT-IR results indicated that glucose oxidase (GOx) could be well immobilized on m-TiO₂. The electrochemical tests of the fabricated Nafion/GOx/m-TiO₂ modified glass carbon electrode (GCE) showed fast direct electron transfer between GOx molecules and electrode surface, exhibiting a linear response to glucose concentration ranging from 0.1 to 1.2 mmol·L^-1, and good sensitivity of 3.44 μA·mmol^-1·L·cm^-2, which proved that the novel m-TiO₂ was a promising material for immobilization of GOx and fabrication of glucose biosensors.