摘要
The effects of fumarase inhibitor cycloheximide on fermentation of L-malic acid and fumaric acid by Rhizopus oryzae ME-M15 were investigated. The results showed that the addition of cycloheximide (15 mg/L) during the acid production stage after 16 h incubation greatly affected the metabolism of Rhizopus oryzae ME-M15. The activity of cytosolic isoenzyme of fumarase was decreased markedly by 48.7%, but the activity of NAD-malate dehydrogenase in the cytosol fraction did not change significantly in the presence of cycloheximide. Fumaric acid production was severely decreased in the presence of cycloheximide with 37.1% in comparison with the control mycelium. In contrast to the inhibitory effect of fumaric acid fermentation, the addition of cycloheximide caused the increase of L-malic acid accumulation to 21.7 g/L, which was 54.6% higher than that of the control. The analyses revealed that the inhibition of fumarase isoenzyme in the cytosol fraction could reduce the metabolic flux from L-malic acid to fumaric acid in the pyruvate carboxylation pathway (cytosolic pathway), promoting the L-malic acid accumulation by Rhizopus oryzae ME-M15.
| 源语言 | 英语 |
|---|---|
| 页（从-至） | 153-156 |
| 页数 | 4 |
| 期刊 | Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering |
| 卷 | 9 |
| 期 | 1 |
| 出版状态 | 已出版 - 2月 2009 |