Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli

Min Jiang; Shu Wen Liu; Jiang Feng Ma; Ke Quan Chen; Li Yu; Fang Fang Yue; Bing Xu; Ping Wei

doi:10.1128/AEM.02190-09

Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli

Min Jiang, Shu Wen Liu, Jiang Feng Ma, Ke Quan Chen, Li Yu, Fang Fang Yue, Bing Xu, Ping Wei

Nanjing Tech University

科研成果: 期刊稿件 › 文章 › 同行评审

64 引用（Scopus）

摘要

Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt snowed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.

源语言	英语
页（从-至）	1298-1300
页数	3
期刊	Applied and Environmental Microbiology
卷	76
期	4
DOI	https://doi.org/10.1128/AEM.02190-09
出版状态	已出版 - 2月 2010

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abstract = "Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt snowed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.",

author = "Min Jiang and Liu, {Shu Wen} and Ma, {Jiang Feng} and Chen, {Ke Quan} and Li Yu and Yue, {Fang Fang} and Bing Xu and Ping Wei",

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AU - Jiang, Min

AU - Liu, Shu Wen

AU - Ma, Jiang Feng

AU - Chen, Ke Quan

AU - Yu, Li

AU - Yue, Fang Fang

AU - Xu, Bing

AU - Wei, Ping

PY - 2010/2

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AB - Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt snowed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.

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Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli

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