TY - JOUR
T1 - Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy
AU - Ji, Xiao Jun
AU - Huang, He
AU - Du, Jun
AU - Zhu, Jian Guo
AU - Ren, Lu Jing
AU - Hu, Nan
AU - Li, Shuang
PY - 2009/7
Y1 - 2009/7
N2 - Batch fermentative production of 2,3-butanediol by Klebsiella oxytoca was investigated using various oxygen supply methods though varying agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (μ), specific glucose consumption rate (qs) and specific 2,3-butanediol formation rate (qp), a two-stage agitation speed control strategy, aimed at achieving high concentration, high yield and high productivity of 2,3-butanediol, was proposed. At the first 15 h, agitation speed was controlled at 300 rpm to obtain high μ for cell growth, subsequently agitation speed was controlled at 200 rpm to maintain high qp for high 2,3-butanediol accumulation. Finally, the maximum concentration of 2,3-butanediol reached 95.5 g l-1 with the yield of 0.478 g g-1 and the productivity of 1.71 g l-1 h-1, which were 6.23%, 6.22% and 22.14% over the best results controlled by constant agitation speeds. Crown
AB - Batch fermentative production of 2,3-butanediol by Klebsiella oxytoca was investigated using various oxygen supply methods though varying agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (μ), specific glucose consumption rate (qs) and specific 2,3-butanediol formation rate (qp), a two-stage agitation speed control strategy, aimed at achieving high concentration, high yield and high productivity of 2,3-butanediol, was proposed. At the first 15 h, agitation speed was controlled at 300 rpm to obtain high μ for cell growth, subsequently agitation speed was controlled at 200 rpm to maintain high qp for high 2,3-butanediol accumulation. Finally, the maximum concentration of 2,3-butanediol reached 95.5 g l-1 with the yield of 0.478 g g-1 and the productivity of 1.71 g l-1 h-1, which were 6.23%, 6.22% and 22.14% over the best results controlled by constant agitation speeds. Crown
KW - 2,3-Butanediol
KW - Batch fermentation
KW - Klebsiella oxytoca
KW - Two-stage control strategy
UR - http://www.scopus.com/inward/record.url?scp=63449119002&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2009.02.031
DO - 10.1016/j.biortech.2009.02.031
M3 - 文章
C2 - 19297148
AN - SCOPUS:63449119002
SN - 0960-8524
VL - 100
SP - 3410
EP - 3414
JO - Bioresource Technology
JF - Bioresource Technology
IS - 13
ER -
