TY - JOUR
T1 - Enhanced photostability of chlorophyll by introducing Mg2Si as an O2-depleting agent
AU - Yi, Zilin
AU - Shan, Zihan
AU - Fang, Liang
AU - Lu, Chunhua
AU - Xu, Zhongzi
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023/4
Y1 - 2023/4
N2 - The application of chlorophyll (Chl) is limited due to its low photostability. The regulation of oxygen status including both oxygen (O2) and singlet oxygen (1O2) around Chl molecules is important to improve their stability. Here, we designed a macroscopic Chl solution system to evaluate the role of magnesium silicide (Mg2Si) as O2-depleting agent in the elimination of dissolved oxygen and singlet oxygen for improving Chl photostability. The results showed that the photostability of Chl solution followed the order of oxygen free (OF) > oxygen blocking (OB) > normoxic (ON) solution conditions upon ultraviolet light irradiation. Based on the oxygen blocking condition, the addition of Mg2Si in Chl solution further created OBC condition that kept Chl in similar oxygen free condition through oxygen blockage and dissolved oxygen consumption. The existence of Mg2Si effectively reduced the content of dissolved oxygen and the generation of singlet oxygen, determined from dissolved oxygen content monitoring, electron spin-resonance spectroscopy and spectroscopic spectroscopy. After 60 min of irradiation, the absorbance retention rate of the Chl solution in the OBC condition reached 68.1%, while the absorbance retention rate of pristine Chl was as low as around 5%. When Mg2Si and β-carotene were added to the Chl solution together, the absorbance retention rate reached 81.6%. This work demonstrated the availability of MgSi2 as O2-depleting agent and provides a new thought to create both O2-depleting and 1O2-depleting environment for improving Chl photostability. Graphical abstract: [Figure not available: see fulltext.].
AB - The application of chlorophyll (Chl) is limited due to its low photostability. The regulation of oxygen status including both oxygen (O2) and singlet oxygen (1O2) around Chl molecules is important to improve their stability. Here, we designed a macroscopic Chl solution system to evaluate the role of magnesium silicide (Mg2Si) as O2-depleting agent in the elimination of dissolved oxygen and singlet oxygen for improving Chl photostability. The results showed that the photostability of Chl solution followed the order of oxygen free (OF) > oxygen blocking (OB) > normoxic (ON) solution conditions upon ultraviolet light irradiation. Based on the oxygen blocking condition, the addition of Mg2Si in Chl solution further created OBC condition that kept Chl in similar oxygen free condition through oxygen blockage and dissolved oxygen consumption. The existence of Mg2Si effectively reduced the content of dissolved oxygen and the generation of singlet oxygen, determined from dissolved oxygen content monitoring, electron spin-resonance spectroscopy and spectroscopic spectroscopy. After 60 min of irradiation, the absorbance retention rate of the Chl solution in the OBC condition reached 68.1%, while the absorbance retention rate of pristine Chl was as low as around 5%. When Mg2Si and β-carotene were added to the Chl solution together, the absorbance retention rate reached 81.6%. This work demonstrated the availability of MgSi2 as O2-depleting agent and provides a new thought to create both O2-depleting and 1O2-depleting environment for improving Chl photostability. Graphical abstract: [Figure not available: see fulltext.].
UR - http://www.scopus.com/inward/record.url?scp=85151540506&partnerID=8YFLogxK
U2 - 10.1007/s10853-023-08398-3
DO - 10.1007/s10853-023-08398-3
M3 - 文章
AN - SCOPUS:85151540506
SN - 0022-2461
VL - 58
SP - 6281
EP - 6296
JO - Journal of Materials Science
JF - Journal of Materials Science
IS - 14
ER -