Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation

Zhaogang Yang; Jing Xie; Jing Zhu; Chen Kang; Chiling Chiang; Xinmei Wang; Xiaobing Wang; Tairong Kuang; Feng Chen; Zhou Chen; Aili Zhang; Bo Yu; Robert J. Lee; Lesheng Teng; L. James Lee

doi:10.1016/j.jconrel.2016.10.008

Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation

Zhaogang Yang, Jing Xie, Jing Zhu, Chen Kang, Chiling Chiang, Xinmei Wang, Xiaobing Wang, Tairong Kuang, Feng Chen, Zhou Chen, Aili Zhang, Bo Yu, Robert J. Lee, Lesheng Teng, L. James Lee

科研成果: 期刊稿件 › 文章 › 同行评审

183 引用（Scopus）

摘要

Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel strategy to produce nanoscale exosome-mimics (EMs) in sufficient quantity for gene delivery in cancer both in vitro and in vivo. Size-controllable EMs were generated at a high yield by serial extrusion of non-tumorigenic epithelial MCF-10A cells through filters with different pore sizes. siRNA was then encapsulated into the EMs by electroporation. Biosafety and uptake efficiency of the EMs were evaluated both in vitro and in vivo. The mechanism underlying their cellular endocytosis was also studied.

源语言	英语
页（从-至）	160-171
页数	12
期刊	Journal of Controlled Release
卷	243
DOI	https://doi.org/10.1016/j.jconrel.2016.10.008
出版状态	已出版 - 10 12月 2016
已对外发布	是

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title = "Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation",

abstract = "Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel strategy to produce nanoscale exosome-mimics (EMs) in sufficient quantity for gene delivery in cancer both in vitro and in vivo. Size-controllable EMs were generated at a high yield by serial extrusion of non-tumorigenic epithelial MCF-10A cells through filters with different pore sizes. siRNA was then encapsulated into the EMs by electroporation. Biosafety and uptake efficiency of the EMs were evaluated both in vitro and in vivo. The mechanism underlying their cellular endocytosis was also studied.",

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author = "Zhaogang Yang and Jing Xie and Jing Zhu and Chen Kang and Chiling Chiang and Xinmei Wang and Xiaobing Wang and Tairong Kuang and Feng Chen and Zhou Chen and Aili Zhang and Bo Yu and Lee, {Robert J.} and Lesheng Teng and Lee, {L. James}",

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T1 - Functional exosome-mimic for delivery of siRNA to cancer

T2 - in vitro and in vivo evaluation

AU - Yang, Zhaogang

AU - Xie, Jing

AU - Zhu, Jing

AU - Kang, Chen

AU - Chiang, Chiling

AU - Wang, Xinmei

AU - Wang, Xiaobing

AU - Kuang, Tairong

AU - Chen, Feng

AU - Chen, Zhou

AU - Zhang, Aili

AU - Yu, Bo

AU - Lee, Robert J.

AU - Teng, Lesheng

AU - Lee, L. James

PY - 2016/12/10

Y1 - 2016/12/10

N2 - Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel strategy to produce nanoscale exosome-mimics (EMs) in sufficient quantity for gene delivery in cancer both in vitro and in vivo. Size-controllable EMs were generated at a high yield by serial extrusion of non-tumorigenic epithelial MCF-10A cells through filters with different pore sizes. siRNA was then encapsulated into the EMs by electroporation. Biosafety and uptake efficiency of the EMs were evaluated both in vitro and in vivo. The mechanism underlying their cellular endocytosis was also studied.

AB - Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel strategy to produce nanoscale exosome-mimics (EMs) in sufficient quantity for gene delivery in cancer both in vitro and in vivo. Size-controllable EMs were generated at a high yield by serial extrusion of non-tumorigenic epithelial MCF-10A cells through filters with different pore sizes. siRNA was then encapsulated into the EMs by electroporation. Biosafety and uptake efficiency of the EMs were evaluated both in vitro and in vivo. The mechanism underlying their cellular endocytosis was also studied.

KW - Electroporation

KW - Endocytosis

KW - Exosome

KW - MCF-7

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=84991798433&partnerID=8YFLogxK

U2 - 10.1016/j.jconrel.2016.10.008

DO - 10.1016/j.jconrel.2016.10.008

M3 - 文章

C2 - 27742443

AN - SCOPUS:84991798433

SN - 0168-3659

VL - 243

SP - 160

EP - 171

JO - Journal of Controlled Release

JF - Journal of Controlled Release

ER -

Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation

摘要

联合国可持续发展目标

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