High-level de novo biosynthesis of cordycepin by systems metabolic engineering in Yarrowia lipolytica

Xi Yu Duan, Yun Tian, Ze Qi Song, Li Ping Song, Wen Bo Lin, Chong Wang, Hui Yang, Xiang Yang Lu, Xiao Jun Ji, Hu Hu Liu

科研成果: 期刊稿件文章同行评审

29 引用 (Scopus)

摘要

Cordycepin is a nucleoside antibiotic with various biological activities, which has wide applications in the area of cosmetic and medicine industries. However, the current production of cordycepin is costly and time-consuming. To construct the promising cell factory for high-level cordycepin production, firstly, the design and construction of cordycepin biosynthetic pathway were performed in Yarrowia lipolytica. Secondly, the adaptivity between cordycepin biosynthetic pathway and Y. lipolytica was enhanced by enzyme fusion and integration site engineering. Then, the production of cordycepin was improved by the enhancement of adenosine supply. Furthermore, through modular engineering, the production of cordycepin was achieved at 3588.59 mg/L from glucose. Finally, 3249.58 mg/L cordycepin with a yield of 76.46 mg/g total sugar was produced by the engineered strain from the mixtures of glucose and molasses. This research is the first report on the de novo high-level production of cordycepin in the engineered Y. lipolytica.

源语言英语
文章编号127862
期刊Bioresource Technology
363
DOI
出版状态已出版 - 11月 2022

指纹

探究 'High-level de novo biosynthesis of cordycepin by systems metabolic engineering in Yarrowia lipolytica' 的科研主题。它们共同构成独一无二的指纹。

引用此