Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15

Bin Zou; Yi Hu; Dinghua Yu; Jiaojiao Xia; Susu Tang; Weiming Liu; He Huang

doi:10.1016/j.bej.2010.09.005

Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15

Bin Zou, Yi Hu, Dinghua Yu, Jiaojiao Xia, Susu Tang, Weiming Liu, He Huang

药学院

Nanjing Tech University

科研成果: 期刊稿件 › 文章 › 同行评审

90 引用（Scopus）

摘要

Ionic liquid was synthesized and grafted onto the surface hydroxyl of mesoporous silica SBA-15 (IL-SBA). Porcine pancreas lipase (PPL) was successfully incorporated into the ionic liquid modified SBA-15 material by physical adsorption (PPL-IL-SBA). The enzymatic properties, including optimum temperature, pH and reusability of PPL-IL-SBA, were investigated in the triacetin hydrolysis reaction. Compared with parent SBA-15 immobilized lipase (PPL-SBA), the enzymatic properties have dramatically improved, especially the optimized activity increased from 594 to 975. U/g PPL after modification.

源语言	英语
页（从-至）	150-153
页数	4
期刊	Biochemical Engineering Journal
卷	53
期	1
DOI	https://doi.org/10.1016/j.bej.2010.09.005
出版状态	已出版 - 15 12月 2010

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10.1016/j.bej.2010.09.005

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title = "Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15",

abstract = "Ionic liquid was synthesized and grafted onto the surface hydroxyl of mesoporous silica SBA-15 (IL-SBA). Porcine pancreas lipase (PPL) was successfully incorporated into the ionic liquid modified SBA-15 material by physical adsorption (PPL-IL-SBA). The enzymatic properties, including optimum temperature, pH and reusability of PPL-IL-SBA, were investigated in the triacetin hydrolysis reaction. Compared with parent SBA-15 immobilized lipase (PPL-SBA), the enzymatic properties have dramatically improved, especially the optimized activity increased from 594 to 975. U/g PPL after modification.",

keywords = "Hydrolysis, Immobilization, Ionic liquid, Lipase, Mesoporous material, Modification",

author = "Bin Zou and Yi Hu and Dinghua Yu and Jiaojiao Xia and Susu Tang and Weiming Liu and He Huang",

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T1 - Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15

AU - Zou, Bin

AU - Hu, Yi

AU - Yu, Dinghua

AU - Xia, Jiaojiao

AU - Tang, Susu

AU - Liu, Weiming

AU - Huang, He

PY - 2010/12/15

Y1 - 2010/12/15

N2 - Ionic liquid was synthesized and grafted onto the surface hydroxyl of mesoporous silica SBA-15 (IL-SBA). Porcine pancreas lipase (PPL) was successfully incorporated into the ionic liquid modified SBA-15 material by physical adsorption (PPL-IL-SBA). The enzymatic properties, including optimum temperature, pH and reusability of PPL-IL-SBA, were investigated in the triacetin hydrolysis reaction. Compared with parent SBA-15 immobilized lipase (PPL-SBA), the enzymatic properties have dramatically improved, especially the optimized activity increased from 594 to 975. U/g PPL after modification.

AB - Ionic liquid was synthesized and grafted onto the surface hydroxyl of mesoporous silica SBA-15 (IL-SBA). Porcine pancreas lipase (PPL) was successfully incorporated into the ionic liquid modified SBA-15 material by physical adsorption (PPL-IL-SBA). The enzymatic properties, including optimum temperature, pH and reusability of PPL-IL-SBA, were investigated in the triacetin hydrolysis reaction. Compared with parent SBA-15 immobilized lipase (PPL-SBA), the enzymatic properties have dramatically improved, especially the optimized activity increased from 594 to 975. U/g PPL after modification.

KW - Hydrolysis

KW - Immobilization

KW - Ionic liquid

KW - Lipase

KW - Mesoporous material

KW - Modification

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DO - 10.1016/j.bej.2010.09.005

M3 - 文章

AN - SCOPUS:78650521855

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JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

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Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15

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