TY - JOUR
T1 - Isolation and characterization of related impurities of monoammonium glycyrrhizinate and development of RP-HPLC method for quality control study
AU - Zou, Qiaogen
AU - Zhan, Ying
AU - Wei, Ping
AU - Ouyang, Pingkai
PY - 2009/1
Y1 - 2009/1
N2 - An HPLC method was developed for the determination of impurities of monoammonium glycyrrhizinate in bulk drugs and pharmaceutical formulations. The separation was accomplished on a Shimadzu Shim-pack C18 (150mm4.6mm, 5m) column. The mobile phase was methanol: (0.2M) ammonium acetate (70:30; v/v, pH=4.5) and a UV detector set at 250nm was used for detection. The unknown impurities were isolated by preparative HPLC and characterized by FT-IR, ESI-MS, 1H NMR and 13C NMR spectral data. The proposed method was validated and successfully applied to the analysis of commercial bulk drugs and formulations, thus it supplied a simple and sensitive method to ensure the quality assurance for the manufactures and researches.
AB - An HPLC method was developed for the determination of impurities of monoammonium glycyrrhizinate in bulk drugs and pharmaceutical formulations. The separation was accomplished on a Shimadzu Shim-pack C18 (150mm4.6mm, 5m) column. The mobile phase was methanol: (0.2M) ammonium acetate (70:30; v/v, pH=4.5) and a UV detector set at 250nm was used for detection. The unknown impurities were isolated by preparative HPLC and characterized by FT-IR, ESI-MS, 1H NMR and 13C NMR spectral data. The proposed method was validated and successfully applied to the analysis of commercial bulk drugs and formulations, thus it supplied a simple and sensitive method to ensure the quality assurance for the manufactures and researches.
KW - Glycyrrhizin
KW - Isolation and characterization
KW - Monoammonium glycyrrhizinate
KW - Related impurities
UR - http://www.scopus.com/inward/record.url?scp=68949144623&partnerID=8YFLogxK
U2 - 10.1080/10826070903091704
DO - 10.1080/10826070903091704
M3 - 文章
AN - SCOPUS:68949144623
SN - 1082-6076
VL - 32
SP - 1953
EP - 1968
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 13
ER -