Kinetic study of asymmetric hydrolysis of dimethyl 3-(4-fluorophenyl) glutarate using novozym 435

Yan Ma, Wei Ming Liu, He Huang, Shuang Li, Yi Hu

科研成果: 期刊稿件文章同行评审

摘要

Immobilized Candida antarctica lipase B (Novozym 435) was used to catalyze the asymmetric hydrolysis of dimethyl 3-(4-fluorophenyl)glutarate (3-DFG) in an aqueous system containing methyl tert-butyl ether (MTBE) as cosolvent. The present work put emphasis on the kinetic study of the asymmetric hydrolysis reaction. The effects of rotation speed, enzyme loading, concentrations of substrate and product on the reaction were investigated. The optimal rotation speed was 200 r·min-1, and the reaction was free from intra-particle diffusion limitations. The inhibitions of substrate (3-DFG) and product (3-MFG) were excluded which both displayed no decline on the activity of Novozym 435 at elevated concentrations within the given range. In addition, methanol, a byproduct of the reaction, inhibited the activity of Novozym 435 following a competitive inhibition pattern. The kinetic constants were obtained through non-linear regression, with values of Km 0.24 mol·L-1, Ki 0.39 mol·L-1, Vmax 2.39 mmol·L-1·h-1, respectively.

源语言英语
页(从-至)145-150
页数6
期刊Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities
29
1
DOI
出版状态已出版 - 1 2月 2015

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