Production of cytidine 5'-diphosphorylcholine with high utilization of ATP by whole cells of Saccharomyces cerevisiae

Jiapeng Tang; Yong Chen; Xiaochun Chen; Yuelan Yao; Hanjie Ying; Jian Xiong; Jianxin Bai

doi:10.1016/j.biortech.2010.06.006

Production of cytidine 5'-diphosphorylcholine with high utilization of ATP by whole cells of Saccharomyces cerevisiae

Jiapeng Tang, Yong Chen, Xiaochun Chen, Yuelan Yao, Hanjie Ying, Jian Xiong, Jianxin Bai

生物与制药工程学院

Nanjing Tech University

科研成果: 期刊稿件 › 文章 › 同行评审

18 引用（Scopus）

摘要

Cytidine 5'-diphosphorylcholine (CDP-choline) was produced using a high efficiency ATP regeneration system and the Kennedy pathway in whole cells of Saccharomyces cerevisiae As 2.398. Out of eight variables, KH₂PO₄, glycerol and (NH₄)₂SO₄ were considered to be the most significant factors by response surface methodology including a Plackett-Burman design, path of steepest accent and central composite design. The optimum levels of the three variables were 20.13g/L KH₂PO₄, 12.35g/L glycerol and 0.49g/L (NH₄)₂SO₄, respectively. Energy utilization efficiency increased from 10.59% to 16.72% and choline chloride conversion yields increased from 12.35% to 42.78%. A high efficiency ATP regeneration system improves CDP-choline production.

源语言	英语
页（从-至）	8807-8813
页数	7
期刊	Bioresource Technology
卷	101
期	22
DOI	https://doi.org/10.1016/j.biortech.2010.06.006
出版状态	已出版 - 11月 2010

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10.1016/j.biortech.2010.06.006

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title = "Production of cytidine 5'-diphosphorylcholine with high utilization of ATP by whole cells of Saccharomyces cerevisiae",

abstract = "Cytidine 5'-diphosphorylcholine (CDP-choline) was produced using a high efficiency ATP regeneration system and the Kennedy pathway in whole cells of Saccharomyces cerevisiae As 2.398. Out of eight variables, KH2PO4, glycerol and (NH4)2SO4 were considered to be the most significant factors by response surface methodology including a Plackett-Burman design, path of steepest accent and central composite design. The optimum levels of the three variables were 20.13g/L KH2PO4, 12.35g/L glycerol and 0.49g/L (NH4)2SO4, respectively. Energy utilization efficiency increased from 10.59% to 16.72% and choline chloride conversion yields increased from 12.35% to 42.78%. A high efficiency ATP regeneration system improves CDP-choline production.",

keywords = "ATP regeneration, Choline chloride, Cytidine 5'-diphosphorylcholine, Saccharomyces cerevisiae",

author = "Jiapeng Tang and Yong Chen and Xiaochun Chen and Yuelan Yao and Hanjie Ying and Jian Xiong and Jianxin Bai",

year = "2010",

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doi = "10.1016/j.biortech.2010.06.006",

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TY - JOUR

T1 - Production of cytidine 5'-diphosphorylcholine with high utilization of ATP by whole cells of Saccharomyces cerevisiae

AU - Tang, Jiapeng

AU - Chen, Yong

AU - Chen, Xiaochun

AU - Yao, Yuelan

AU - Ying, Hanjie

AU - Xiong, Jian

AU - Bai, Jianxin

PY - 2010/11

Y1 - 2010/11

N2 - Cytidine 5'-diphosphorylcholine (CDP-choline) was produced using a high efficiency ATP regeneration system and the Kennedy pathway in whole cells of Saccharomyces cerevisiae As 2.398. Out of eight variables, KH2PO4, glycerol and (NH4)2SO4 were considered to be the most significant factors by response surface methodology including a Plackett-Burman design, path of steepest accent and central composite design. The optimum levels of the three variables were 20.13g/L KH2PO4, 12.35g/L glycerol and 0.49g/L (NH4)2SO4, respectively. Energy utilization efficiency increased from 10.59% to 16.72% and choline chloride conversion yields increased from 12.35% to 42.78%. A high efficiency ATP regeneration system improves CDP-choline production.

AB - Cytidine 5'-diphosphorylcholine (CDP-choline) was produced using a high efficiency ATP regeneration system and the Kennedy pathway in whole cells of Saccharomyces cerevisiae As 2.398. Out of eight variables, KH2PO4, glycerol and (NH4)2SO4 were considered to be the most significant factors by response surface methodology including a Plackett-Burman design, path of steepest accent and central composite design. The optimum levels of the three variables were 20.13g/L KH2PO4, 12.35g/L glycerol and 0.49g/L (NH4)2SO4, respectively. Energy utilization efficiency increased from 10.59% to 16.72% and choline chloride conversion yields increased from 12.35% to 42.78%. A high efficiency ATP regeneration system improves CDP-choline production.

KW - ATP regeneration

KW - Choline chloride

KW - Cytidine 5'-diphosphorylcholine

KW - Saccharomyces cerevisiae

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U2 - 10.1016/j.biortech.2010.06.006

DO - 10.1016/j.biortech.2010.06.006

M3 - 文章

C2 - 20620046

AN - SCOPUS:77955175898

SN - 0960-8524

VL - 101

SP - 8807

EP - 8813

JO - Bioresource Technology

JF - Bioresource Technology

IS - 22

ER -

Production of cytidine 5'-diphosphorylcholine with high utilization of ATP by whole cells of Saccharomyces cerevisiae

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