TY - JOUR
T1 - Production of fumaric acid by simultaneous saccharification and fermentation of starchy materials with 2-deoxyglucose-resistant mutant strains of Rhizopus oryzae
AU - Deng, Yuefang
AU - Li, Shuang
AU - Xu, Qing
AU - Gao, Min
AU - Huang, He
PY - 2012/3
Y1 - 2012/3
N2 - A mutant strain with high glucoamylase activity and insensitive to catabolite repression was developed to produce fumaric acid by simultaneous saccharification and fermentation (SSF) of starch without additional commercial glucoamylase supplementation. A series of mutant strains resistant to the non-metabolizable and toxic glucose analog 2-deoxyglucose (2-DG) were obtained by implanting nitrogen ion (N +) into Rhizopus oryzae ME-F12. Among them, the best mutant strain DG-3 produced 39.80g/L fumaric acid, which is 1.28-fold of that produced by ME-F12, and exhibited higher glucoamylase activity during SSF. Higher fumaric acid production (44.10g/L) was achieved when the initial total sugar concentration of cornstarch was 100g/L. During SSF of cheap, raw bioresource-degermed corn powder (100g/L total sugar) by DG-3, the maximum fumaric acid concentration and productivity were 32.18g/L and 0.44g/(Lh), respectively.
AB - A mutant strain with high glucoamylase activity and insensitive to catabolite repression was developed to produce fumaric acid by simultaneous saccharification and fermentation (SSF) of starch without additional commercial glucoamylase supplementation. A series of mutant strains resistant to the non-metabolizable and toxic glucose analog 2-deoxyglucose (2-DG) were obtained by implanting nitrogen ion (N +) into Rhizopus oryzae ME-F12. Among them, the best mutant strain DG-3 produced 39.80g/L fumaric acid, which is 1.28-fold of that produced by ME-F12, and exhibited higher glucoamylase activity during SSF. Higher fumaric acid production (44.10g/L) was achieved when the initial total sugar concentration of cornstarch was 100g/L. During SSF of cheap, raw bioresource-degermed corn powder (100g/L total sugar) by DG-3, the maximum fumaric acid concentration and productivity were 32.18g/L and 0.44g/(Lh), respectively.
KW - Cornstarch
KW - Degermed corn powder
KW - Fumaric acid
KW - Rhizopus oryzae
KW - Simultaneous saccharification fermentation (SSF)
UR - http://www.scopus.com/inward/record.url?scp=84856581606&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2011.11.117
DO - 10.1016/j.biortech.2011.11.117
M3 - 文章
C2 - 22217732
AN - SCOPUS:84856581606
SN - 0960-8524
VL - 107
SP - 363
EP - 367
JO - Bioresource Technology
JF - Bioresource Technology
ER -
